透明颤菌血红蛋白基因克隆及在紫色非硫细菌中的表达

Cloning of vitreoscilla hemoglobin gene and expression in Rhodopseudo palustns

构建了含有透明颤菌血红蛋白基因的可转化大肠杆菌-紫色非硫细菌穿梭质粒（pHSG-MG-vgb），将其转化到紫色非硫细菌（Rhodopseudo palustns），获得了基因工程菌EPB01。经SDS-PAGE和CO示差分光光谱分析证明，重组菌株表达了有活性的透明颤菌血红蛋白，通过低溶解氧诱导后，基因工程菌EPB01的菌体密度较对照菌增加了24%-32%。

An E. coli-Rhodopseudo palustns shuttle plasmid, pHSG-MG-vgb containing vitreoscilla hemoglobin gene was constructed and transformed to Rhodopseudo palustns. As a result genetic Engineering strains EPB01 was obtained. After inducing under low dissolved oxygen conditions, the density of engineering strain increased by 24%-32% compared with that of the control group. The results of SDS-PAGE and carbon monoxide （CO） difference spectrum analysis indicated that the recombinant strain expressed vitreoscilla hemoglobin （VHb） having biological activity.