摘要
采用正交分析方法对冬虫夏草菌丝体的液体培养基配方进行了优化,结果表明,冬虫夏草生长最适液体培养基配方为:葡萄糖1.25%,蔗糖1.25%,蛋白胨0.02%,酵母粉0.0625%,KH2PO4 0.025%,MgSO4·7H2O 0.0125%,VB10.0025%,pH自然;培养温度24℃,周期192h,其菌丝体生物量产率为19.5g/L,是前人菌丝体生物量产率的1.2~1.5倍.
The liquid culture media of Cordyceps sinensis mycelia was optimized by means of orthogonal analysis. The results showed that the optimal medium for the mycelia was 1.25% glucose, 1.25% sucrose, 0.02% peptone, 0. 062 5% yeast powder, 0. 025% KH2PO4, 0. 012 5% MgSO4· 7H2O, 0, 002 5% VB] and pH natural. In the cultural condition of 24℃ and 192 h, the mycelia biomass reached to 19.5 g/L, which was as 1.2~1.5 times as the amount with the previous way.
出处
《天津师范大学学报(自然科学版)》
CAS
2008年第1期8-11,共4页
Journal of Tianjin Normal University:Natural Science Edition
基金
天津市科学技术委员会资助项目(06ZHCXNC05900)
关键词
正交分析
冬虫夏草
菌丝体
液体培养基
优化
生物量
orthogonal analysis
Cordyceps sinensis
mycelia
liquid culture media
optimize
biomass