摘要
采用正交分析方法对冬虫夏草菌丝体的液体培养基配方进行了优化，结果表明，冬虫夏草生长最适液体培养基配方为：葡萄糖1．25％，蔗糖1．25％，蛋白胨0．02％，酵母粉0．0625％，KH2PO4 0．025％，MgSO4·7H2O 0．0125％，VB10．0025％，pH自然；培养温度24℃，周期192h，其菌丝体生物量产率为19．5g／L，是前人菌丝体生物量产率的1．2～1．5倍．
The liquid culture media of Cordyceps sinensis mycelia was optimized by means of orthogonal analysis. The results showed that the optimal medium for the mycelia was 1.25% glucose, 1.25% sucrose, 0.02% peptone, 0. 062 5% yeast powder, 0. 025% KH2PO4, 0. 012 5% MgSO4· 7H2O, 0, 002 5% VB] and pH natural. In the cultural condition of 24℃ and 192 h, the mycelia biomass reached to 19.5 g/L, which was as 1.2～1.5 times as the amount with the previous way.
出处
《天津师范大学学报（自然科学版）》
CAS
2008年第1期8-11,共4页
Journal of Tianjin Normal University：Natural Science Edition
基金
天津市科学技术委员会资助项目(06ZHCXNC05900)
关键词
正交分析
冬虫夏草
菌丝体
液体培养基
优化
生物量
orthogonal analysis
Cordyceps sinensis
mycelia
liquid culture media
optimize
biomass
