三种肺炎支原体检测方法的比较

Diagnosis of Mycoplasma Pneumoniae in Community Acquired Pneumoniae by Comparision with Three Different Methods

目的比较3种检测肺炎支原体(mycoplasma pneumoniae,Mp)的方法,寻求肺炎支原体的快速、准确和特异的检测手段。方法选择198例社区获得性肺炎(CAP)患者中临床证实的45例肺炎支原体感染者的痰液、血清标本,分别经肺炎支原体培养、肺炎支原体核酸PCR扩增和血清学检测(IgM和IgG),采用χ2检验比较3种方法的检测结果。结果3种方法检测Mp的阳性率分别为44.4%、71.1%和86.7%和,PCR法与培养法比较差异有显著性(χ2=17.764,P<0.001);血清学与培养法比较差异有显著性(χ2=6.559,P<0.01);PCR法与血清学比较差异无显著性(χ2=3.269,P=0.071)。结论PCR法和血清学检测是肺炎支原体病原体诊断的有效手段。

Objective To explore a rapid and accurate method for Mycoplasma pneumoniae with community aquired pneumoniae. Methods 45 sera and sptum of patients with CAP were tested hy culture,Mp selective P medium hy PCR,Mp specific IgM and IgG by ELISA. The results were analyzed by chi square test. Results The positive rate of three methods for detecting Mycoplasma pneumoniae were 44.4%, 71.1% and 86.7% respectively. The result was different significantly by comparing the test of PCR with one of culture （Х^2= 17. 764,P〈0. 001）. The same results were analyzed between the positive rates of serum and culture （Х^2=6. 559,P〈0. 01 ）. Nevertheless, there was not difference between positive rate of PCR and serum sample tests（Х^2=3. 269,P=0. 071）. Conclusion Tests of analyzing IgG , IgM in serum and PCR were effective for Mycoplasma pneumoniae.