基质金属蛋白酶-9和血管内皮生长因子在膀胱移行上皮细胞癌中的表达及其临床意义

The expressions of MMP-9 & VEGF and the clinical significance in bladder transitional epithelial cell carcinoma

目的探讨基质金属蛋白酶-9(MMP-9)和血管内皮生长因子(VEGF)在膀胱移行上皮细胞癌(BTCC)中的表达及其临床意义。方法采用免疫组织化学SP法检测46例BTCC和10例正常膀胱黏膜组织中MMP-9和VEGF的表达。结果MMP-9和VEGF的阳性表达率在BTCC组中分别为76.09%和67.39%,均显著高于正常组,两组比较差异有统计学意义(P<0.01);BTCC中两种蛋白表达水平在高分化组与中低分化组之间、Tis-T1期与T2-T4期间的差异均有统计学意义(P<0.05)。MMP-9和VEGF两者表达呈正相关。结论MMP-9和VEGF与膀胱移行上皮细胞癌的恶性程度有关,并可作为研究膀胱肿瘤的生物学行为和判断预后的参考指标。

Objective To study the expressions and clinical significance of matrix metalloproteinase 9 （MMP-9） and vascular endothelial growth factor （VEGF） in bladder transitional epithelial cell carcinoma. Methods Immunohistochemical SP method was used to study the expressions of MMP-9 and VEGF in 46 cases of bladder transitional epithelial cell carcinoma and 10 cases of normal bladder tissues. Results The expression rate of MMP-9 and VEGF in bladder carcinoma was 76.09 % and 67.39 % respectively, which was higher than and different from that in the normal group （non-tumor bladder group） （P〈0.01）. And the expressions of MMP-9 and VEGF were different when the well differentiated bladder cancer was compared with the moderately and poorly differentiated bladder cancer （P〈0.05） and were different in the grade of Tis-T1 and T2 -T4 （P〈0.01）. MMP-9 and VEGF were positive correlated. Conclusion MMP-9 and VEGF were related to malignant grade of BTCC, The investigation of MMP-9 and VEGF in bladder transitional cell carcinoma may provide some experimental basis for evaluating biological behaviour of bladder tumor and prognosis.