肾癌患者外周血中survivin mRNA的表达

Detection and clinical significance of survivin mRNA from peripheral blood cells by reverse transcriptase-polymerase chain reaction in patients with renal cell cancer

目的探讨肾透明细胞癌患者外周血中survivin mRNA的表达及其临床意义。方法用RT-PCR方法检测30例肾透明细胞癌患者、10例正常健康人外周血中survivin mRNA的表达。结果30例肾透明细胞癌患者中有23例外周血中survivin mRNA表达阳性,阳性率为76.7%,与正常健康人(0%)相比差异有统计学意义(P<0.001);survivin mRNA的表达与临床分期密切相关(P<0.01);随肿瘤组织分化程度的降低,survivin阳性率有增加的趋势,但差异无统计学意义(P>0.05)。结论肾透明细胞癌患者外周血中survivin mRNA可作为肾透明细胞癌微转移的一个监测指标。

Objective To investigate the survivin mRNA expression in peripheral blood cells from patients with renal cell cancer and to evaluate its clinical significance. Methods Peripheral blood samples were collected from 30 eligible patients with renal cell cancer and 10 normal persons. Survivin mRNA was detected by reverse transcriptase polymerase chain reaction （RT-PCR）. Results The positive rate of Survivin mRNA in blood samples of the 30 patients was 76.7 % and there were significant differences compared with healthy volunteers （P〈0. 001）. The expression of Survivin mRNA was related to the clinical stage （P〈0.01）. The positive rate increased with the pathological grade but the expression of Survivin mRNA was not correlated with it （P〉0.05）. Conclusion The expression of survivin mRNA in peripheral blood cells correlates with the clinical staging of renal cell cancer and it can be an effective signal to monitor the micrometastases of tumor.