细胞黏附分子与子痫前期发病关系的研究

Study on Pre-eclampsia's Relationship with Cell Adhesions Molecule

目的检测子痫前期患者和正常孕妇胎盘组织中血管细胞黏附分子-1(VCAM-1)、细胞间黏附分子-1(ICAM-1)及血小板内皮细胞黏附分子-1(PECAM-1)的表达水平,以探讨细胞黏附分子(CAM)在子痫前期发病机制中的作用。方法选取2005年5月-2006年5月在郑州大学第三附属医院住院分娩病例80例,分为3组:①轻度子痫前期组20例;②重度子痫前期组30例;③正常晚孕组(NT组)30例;胎盘组织中MCAM-1、ICAM-1及PECAM-1的表达水平采用免疫组化PV-9000法测定。结果①重度子痫前期组胎盘组织中VCAM-1和PECAM-1的表达下降(P<0.05),ICAM-1的表达增强(P<0.05)。②轻度子痫前期组胎盘组织中VCAM-1的表达下降(P<0.05)。ICAM-1和PECAM-1的表达无明显差异(P<0.05)。结论CAM与子痫前期发病有密切联系,VCAM-1、ICAM-1和PECAM-1参入了子痫前期病理生理过程且与子痫前期的病情程度密切相关。

Objective To evaluate the expression of ICAM - 1 VCAM - 1 and PECAM - 1 in the placenta tissue in women with preeclampsia, and to discuss the role of CAM in the etiology of preeclampsia. Methods The subjects 80 cases were divided into three groups ①light preeclampsia group （ n = 20） . ②severe preeclampsia group （ n = 30） . ③normal group （ n = 30） . PV - 9000 Polymer Detection System was applied to detect the expression of VCAM - 1, ICAM - 1 and PECAM - 1 in the placenta tissue. Results ④The levels of VCAM - 1 and PECAM - 1 expression in placenta of severe preeclampsia patients were low （ P 〈 0. 05）, and ICAM - 1 was higher than NT （ P 〈 0. 05）. ⑤ The level of VCAM - 1 expression in placenta of light preeclampsia patients was low （ P 〈 0. 05 ）, and that of ICAM - 1 and PECAM - 1 were no difference （ P 〈 0. 05）. Conclusion VCAM - 1, ICAM - 1 and PECAM -1 expression in placenta cytotrophoblast play a role in peeclampsia and they are closely related to the stages of preeclampsia.