ELISA法定量测定抗精子抗体AsAb

Quantitative Measurement of Total Antisperm Antibody by ELISA Method

目的 通过对抗精子总抗体（AsAb）的测定,探讨其与临床不育（或不孕）症的关系。方法 对107例志愿者AsAb的类型和含量用ELISA法进行了分型和定量研究。结果 总抗体的测定上限可达500U/ml;回收率在96.4%～103.0%之间;批内重复性为2.1%～5.0%;本试剂盒呈色稳定性较好,操作简单。通过测定其参考值范围为AsAb〈75.3U/ml。结论 该法准确可靠,可作为临床诊断不育或不孕症病因的辅助诊断方法 。

Objective To elucidate the relationship of antisperm antibody （AsAb） - total, with infertility clinic by measurement of their levels. Method The types and content of AsAb in 107 volunteers were classified and quantified with ELISA. Results The upper limit of AsAb was measured near 500U/rot; the recycle rate was between 96. 4% and 103.0% ;the repeatability in a batch was 2. 1% -5.0%. And the reagent box has better color stability, and it is easy to operate. The range of reference value detemlined was AsAb 〈75.3U/ml. Conclusion The method is accurate and dependable, so it may be as a assistant method of clinical diagnosis of pathogen of infertility.