红细胞吸附释放法纯化新城疫病毒

Purification of Newcastle Disease Nirus(NDV) with Absorption and Release-RBC Method

制备50%的醛化红细胞,将新城疫病毒(NDV)感染的鸡胚尿囊液采用醛化红细胞吸附释放法进行纯化,然后分别对纯化的病毒液及弃去的上清进行血凝检测,并与差速离心法进行比较,结果表明:使用50%的醛化红细胞基本能将病毒全部吸附,SDS-PAGE电泳显示其纯度较高,因此醛化红细胞吸附释放法是一种经济实用、操作简便,并有较高纯化效率的方法.

The concentration of 50% red blood cells （RBC） treated by formaldehyde is prepared, a method of RBC absorption-release vires is used to purify NDV in aUantoic fluid and the HA titers of precipitation and supemate are examined respectively. This method is compared with differential speed centrifugation method. The result shows the concentration of 50% RBC treated by formaldehyde can effectively absorb the virions with higher purity identified by SDS-PAGE electrophoresis. Compared differential speed centrifugation method, using red blood cells treated by formaldehyde, therefore, is an efficient, economical and available method.