VK2对肝癌细胞株MHCC97H的抑制作用

Inhibitory effects of vitamin K2 on the highlv metastatic cell line MHCC97-H of human hepatocellular carcinoma

目的观察 VK2对 MHCC97H 细胞在生长、黏附、侵袭、凋亡以及 Caspase-3活性的影响。方法噻唑蓝(MTT)法检测细胞生长;体外细胞黏附及侵袭实验检测细胞的黏附和侵袭能力;Annexin V-FITC Apoptosis Detection K 和 Caspase-3 Fluorescent Assay 试剂盒分别检测细胞的凋亡及 Caspase-3活性。结果当 VK2浓度从10^(-7)mol/L 增加到10^(-4)mol/L 时,细胞生长抑制率由12.5%增加到59.7%(P<0.01)。VK2对细胞的黏附抑制能力呈剂量依赖关系(P<0.01);100μmol/L的 VK2作用细胞3 h 后,细胞对 Fibronectin 黏附抑制率为69.9%。作用48 h 后,细胞侵袭抑制率为65.5%(P<0.01);作用6 h 后,细胞凋亡率为(28.5±1.6)%,与此同时,细胞 Caspase-3活性为(226.0±6.4),与对照组(92.0±5.8)比较差异有统计学意义(P<0.01);预先加入 Caspase-3抑制剂(Z-VAD-FMK)后,Caspase-3活性无明显变化90.0±4.3(P>0.05)。结论 VK2对体外培养的 MHCC97H 细胞具有抑制生长、抑制黏附、抑制侵袭和诱导凋亡作用;Caspase-3参与了细胞凋亡的调控。

Objective To verify whether vitamin K2 （VK2） has a capacity of inhibiting proliferation, adhesion and invasiveness and inducing apoptosis in a HCC cell line with high metastatic potential （MHCC97-H） ,and also to investigate the Caspase-3 activity in the process of apoptosis induced by VK2. Methods After MHCC97-H cells were treated with 0-100 μM VK2, cell adhesion and migration potential were tested by in vitro assay of cell adhesion and invasion ; cell proliferation by MTT assay; and apoptosis and Caspase-3 activity by Annexin V-FITC Apoptosis Detection Kit and Caspase-3 Fluorescent Assay Kit, respectively. Results VK2 inhibited MHCC97-H cell proliferation from 12.5% to 59.7% when the dose was increased from 10.7 mol/L to 10-4 mol/L （ P 〈 0.01 ）. VK2 in 100 μmol/L markedly inhibited cell adhesion to fibronectin （FN） up to 69.9% in a dose-dependent manner following 3 h of treatment （ P 〈 0.01 ）,and the invasiveness was significantly inhibited up to 65.5% following 48 h of treatment （P 〈 0.01 ）. After treatment with VK2 in 100 μmol/L for 6 h ,28.5% of the cells exhibited apoptotic traits, and in the meantime, Caspase-3 activity of MHCC97-H cells was increased significantly from 92.0 ± 5.8 to 226.0 ± 6.4 （P 〈 0.01 ）. The inhibitor of Caspase-3, Z-VAD-FMK, was found to have the ability to limit Caspase-3 activity. Conclusion VK2 produced marked inhibitory effects on the adhesion, invasion and proliferation of MHCC97-H cells, and the apoptosis of MHCC97-H cells could be induced by VK2 via C aspase-3 -transduction signal.