雷公藤多甙对白细胞介素-1β诱导大鼠滑膜细胞株RSC-364基质金属蛋白酶-3、c-Jun基因表达的影响

The effect of tripterygium glycosides on the expression of MMP3 and c-Jun mRNA in rat synovial cell line RSC-364 stimulated with IL-1β

目的 观察雷公藤多甙对白细胞介素（IL）-1β诱导大鼠滑膜细胞株RSC-364基质金属蛋白酶-3（MMP3）、c—Jun mRNA表达的影响。方法 选择不同浓度的雷公藤多甙作用IL-1β刺激的大鼠滑膜细胞株RSC-364，37℃，5％CO2的环境下培养48h，运用酶联免疫吸附试验（ELISA）检测细胞上清的MMP3和实时聚合酶链反应（real—time PCR）检测分析细胞中的c—Jun mRNA表达。结果 不同浓度的雷公藤多甙（0、5、10、20mg／L）对IL-1β刺激的大鼠滑膜细胞株RSC-364分泌MMP3的水平依次是198、176、140、115μg／L，c—Jun mRNA表达分别是1．17×10^6、3．31×10^5、9．32×10^4、2．81×10^4 copies／ml，即呈现明显地抑制。重要的是：c—Jun mRNA表达抑制呈现明显的剂量依赖性。结论雷公藤多甙治疗类风湿性关节炎的有效的作用机制，可能与其抑制MMP3、c—Jun基因的表达相关。

Objective To explore the effect of tripterygium glycosides （TG） on theexpression of matrix metalloproteinase -3 （MMP3） and c-Jun mRNA in rat synovial cell line RSC-364 stimulated with interleukin （ IL）-1β （IL-1β） and to illuminate the molecular mechanism of TG in the treatment of rheumatoid arthritis （RA）. Methods The rat synvoial cell line RSC-364 stimulated with IL-1β （ 10μg/L） was induced by different doses of TG （0,5,10 and 20 mg/L） and at 37℃ under 5% CO2 atmosphere was incubated for 48 h. MMP3 in cell supernatant was measured using enzyme-linked immunosorbent assays and the expression of c-Jun mRNA was detected by using real-time reverse transcription polymerase chain reaction. Results The expression of MMP3 in the rat synovial cell line RSC-364 stimulated with IL-1βwas 198,176,140,115 μg/L respectively by different doses of TG （0,5,10 and 20 mg/L）,and the expression of e-Jun mRNA was 1. 17× 10^6, 3.31 × 10^5, 9.32×10^4, 2.81 ×10^4 copies/ml respectively, which were suppressed obviously; Of note ,the suppression of the expression of c-Jun mRNA was dose-dependant. Conclusion The effective mechanism of TG in the treatment of RA might be related with the suppression of the expression of MMP3 and c-Jun mRNA.