摘要
目的：研究麝香酮对PCl2细胞缺血损伤的影响。方法：在离体培养的PCl2细胞，用NaCN加缺糖造成拟缺血损伤．模型，通过细胞形态学观察、MTT微量比色、培养介质LDH活力测定，研究了麝香酮对该模型的保护作用。结果：10^-7～5 10^-5mol／L范围内，麝香酮呈浓度依赖性地降低NaCN加缺糖造成拟缺血损伤模型培养介质内LDH的释放，其IC50为：43．33μmol／L。在10^-7～5 10^-5mol／L范围内，麝香酮呈浓度依赖性地增加NaCN加缺糖造成拟缺血损伤模型培养介质的MTT比色值，其IC50为30.62μmol／L。结论：麝香酮对PCl2细胞缺血损伤具有保护作用。
Objective： To investigate the effects of muskiness ketone on ischemic injury in cultured PCl2 cells. Methods： Cultured PCl2 cells were treated with 20mol/L NaCN in combination with glucose deprivation. The protective effects of muskiness ketone on the model was evaluated by lactate dehydrogenate （ LDH ） efflux assay and colormetric MTT assay. Results ： Muskiness ketone, within the range of 10^-7 - 5 × 10^-5 mol/L significantly antagonized LDH efflux induced by the model and increase the optical density at 570nm tested by colorimetric MTT assay in concentration-dependent manner. Conclusion： It was shown that muskiness ketone markedly protected PCl2 cells from ischemic injury.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2007年第6期8-10,共3页
Pharmacology and Clinics of Chinese Materia Medica
基金
山东省自然科学基金项目(Y2003C02)
