加味玉屏风散对创伤应激小鼠免疫功能抑制的影响

Study on the immunoregulatory effects of Jiawei Yupingfeng Powder (加味玉屏风散) in mice with traumatic stress

目的:研究加味玉屏风散对创伤应激小鼠免疫功能调节作用的影响。方法:采用小鼠截肢应激模型,用流式细胞仪检测小鼠脾细胞悬液中CD4+CD25-T、CD4-CD25+T、CD4+CD25+Tr的细胞数,ELISA法检测小鼠脾细胞上清液中γ-干扰素(IFN-γ)、转化生长因子-β(TGF-β)水平。结果:创伤应激后,IFN-γ水平、CD4+CD25-T、CD4-CD25+T细胞比例明显降低,TGF-β水平和CD4+CD25+Tr细胞比例明显升高;口服加味玉屏风散能提高IFN-γ水平和CD4+CD25-T、CD4-CD25+T细胞比例,降低TGF-β水平及CD4+CD25+Tr细胞比例。结论:创伤后小鼠免疫功能受抑,加味玉屏风散可恢复其免疫功能。

Objective： To investigate the immunoregulatory effects of Jiawei Yupingfeng Powder（JYP） in mice with traumatic stress. Methods： The model of traumatic stress in mice was induced by amputation,detected the percentage and ratio of T cell with CD4 ^＋ CD25 ,CD4^＋CD25 ^＋and CD4 ^＋ CD25 ^＋ regulatory T cell in splenic suspension by flow cytometry （FCM） , the levels of IFN-γ and TGF-β in splenic supernatant were determined by enzyme-linked immunosorhent assay （ELISA）, Result： After traumatic stress, the level of IFN-γ and the ratio of T cell with CD4 ^＋ CD25^- ,CD4^-CD25 ^＋ were markedly decreased; the TGF-β level and the ratio of CD4 ^＋ CD25 ^＋ regulatory T cell were increased ; administration with JYP significantly increased the level of IFN-γ and the ratio of T cell with CD4 ^＋ CD25^＋ ,CD4^-CD25 ^＋ ,reduced the TGF-β level and the ratio of CD4 ^＋ CD25 ^＋ regulatory T cell. Conclusion： JYP can significantly alleviate the immunosuppressive response induced by traumatic stress in mice.