过继转移FasL基因修饰的树突细胞对小鼠自然流产模型胚胎丢失的影响

The influence of adoptive transfer of FasL-DC on the embryo loss in the CBA/J×DBA/2 mice model.

目的：观察过继转移FasL基因修饰的树突细胞（DC）对小鼠自然流产模型胚胎丢失的影响，探讨它在诱导妊娠免疫耐受中的作用。方法：构建鼠源FasL（mFasL）真核表达载体pcDNA3．1-mFasL，用电转染法将它转染给DBA／2雄鼠骨髓来源的DC，将转染成功的mFasL-DC于交配前经腹腔注射给CBA／J母鼠。实验动物分为6组：（1）正常妊娠模型组（CBA／J×BALB／c）；（2）未添加干预的流产模型组（CBA／J×DBA／2）；（3）转输DC培养基（DCCM）的流产模型组；（4）转输单纯DC（DC）的流产模型组；（5）转输转染空质粒DC的流产模型组；（6）转输转染mFasL质粒DC的流产模型组，于妊娠第12～14天观察孕鼠胚胎丢失率。结果：转输mFasL—DC后孕鼠胚胎丢失率明显低于未添加干预或转输DC培养基的流产模型组（P〈0．01），与转输单纯DC或带空质粒的DC组相比，其胚胎丢失率也明显下降（P〈0．05），它与正常妊娠组相比胚胎丢失率无显著差异（P〉0．05）；转输单纯DC或空质粒的DC组与未添加干预流产模型组相比胚胎丢失率有所下降，但没有统计学差异；转输DC培养基组与未加干预组之间胚胎丢失率无统计学差异（P〉0．05）。结论：过继转移mFasL-DC能诱导妊娠免疫耐受，降低小鼠自然流产模型孕鼠胚胎丢失率。

Objective：To investigate the effect of FasL-DC on the embryo loss in the CBA/J × DBA/2 abortion mice model. Methods： Constructed the eukaryotic expression vector pcDNA3.1-mFasL,then transfected it into DC cells deriving from bone marrow of DBA/2 mice. CBA/J females were inoculated with mFasL-DC twice before mating. Six different experimental groups were included ： （ 1 ） mice of normal pregnancy （ CBA/J ~ BALB/c ） were served as con- trois; （2）no treatment control（ CBA/J x DBA/2） ; （3） mice injected with DC cells culture me- dium（DCCM） （CBA/J ~ DBA/2 ） ; （3） immunized with DC （CBA/J ~ DBA/2 ） ; （4） immu- razed with empty plasmid pcDNA3.1-DC （CBA/J ~ DBA/2） ; （5） immunized with pcDNA3.1- FasL-DC（ CBA/J ~ DBA/2） ,observated embryo loss rate on day 12 to 14 of gestation. Results： The embryo loss rate of inoculation with mFasL-DC was decreased significantly as compared with that of no treatment control and DCCM group（ P 〈 0.01 ）, and the embryo loss rate were also decreased compared with that of inoculating with DC and pcDNA3. 1-DC group （P 〈 0. 05 ）, there were no significant difference between FasL-DC group and normal pregnancy group（P 〉 0.05 ）. Compared with that of no treatment control group, the embryo loss rate of inoculating with DC or with pcDNA3. 1-DC were decreased, but there was no statistical difference. The embryo loss rate of DCCM group were no significant difference with that of no treatment. Conclusion： Adoptive transfer of mFasL-DC can decrease significantly the embryo loss rate of abortion model mice.