川芎嗪对子痫前期脐血清诱导脐静脉内皮细胞NF-κB活性及VCAM-1表达的影响

The effects of ligustrazine on activation of NF-κB and expression of VCAM-1 in cultured human umbilical vascular endothelial cell induced by sera of preeclampsia.

目的：探讨川芎嗪对子痫前期患者脐静脉血清诱导脐静脉内皮细胞（HU—VEC）核因子-κB（NF-κB）活性及其靶基因血管细胞黏附分子-1（VCAM-1）表达变化的影响。方法：用胰酶消化法培养正常妊娠HUVEC，传代后待细胞长满至70％～80％，加或不加川芎嗪作用30min后分别加入正常妊娠及子痫前期脐静脉血清，培养48h后，MTT测定细胞活力，流式细胞学测定细胞凋亡率，Western-blot测定HUVECNF-κB的表达，酶联免疫检测VCAM-1的表达。结果：子痫前期患者脐静脉血清培养HUVEC的增殖活力明显低于正常妊娠组，胞核NF-κB及VCA-1表达明显高于正常妊娠组（P〈0．01），子痫前期患者脐静脉血清培养HUVEC的细胞凋亡率明显高于正常妊娠组（P〈0．01）。加川芎嗪预处理后，子痫前期组HUVEC增殖活力明显增加，细胞凋亡率、胞核NF-κB及VCAM-1表达明显下降（P〈0．01）。结论：川芎嗪可抑制子痫前期患者脐静脉血清诱导的HU-VEC凋亡，NF-κB的核转位及VCAM-1的表达。

Objective：To study the effects of ligustrazine （LTZ） on the nuclear factor kappa-B （NF-κB） activation and the expression of vascular cell adhesion molecules-1 （ VCAM- 1 ） of cultured human umbilical vascular endothelial cell （HUVEC） induced by preeclamptic sera. Methods： HUVEC were incubated with preeclamptic sera in the presence or in the absence of LTZ. The express;on of cytoplasmic I-κB, nuclear NF-κB p65 were detected by Westem blotting after HUVEC was incubated for 2hours. The apoptosis of HUVEC were analyzed by flow cytomerty, and the expression of VCAM-1 was determined using ELISA after HUVEC was incubated for 48hours. Results： Preeclamptic sera could stimulate the apoptosis , the activation of NF-κB ,and the expressions of VCAM-1 of HUVEC as compared with normal umbilical sera（ P 〈0.01 ）. LTZ could inhibit the apoptosis , the activation of NF-κB, and the expressions of VCAM-1 of HUVEC stimulated by preeclamptic sera（ P 〈 0.01 ）. Conclusion： LTZ can inhibit the activation of NF-κB and the expressions of VCAM-1 of HUVEC stimulated by preeclamptic sera.