中国辽宁地区人群幽门螺杆菌感染菌株与相关性胃疾病的关系

Relationship between Helicobacter pylori infection and associated gastric diseases in Liaoning Province of China

目的:探讨中国辽宁地区人群H pylori致病基因的疾病相关性,为揭示H pylori的致病机制及监测H pylori相关性胃疾病的高危人群提供线索.方法:选取胃黏膜活检标本491例,分组浅表性胃炎(GS)、萎缩性胃炎(GA)、溃疡(GU)、胃癌(GC),在微需氧的条件下,培养出H pylori 222例,并用标准的酚-氯仿方法提取菌种DNA后经聚合酶链反应及琼脂糖凝胶电泳对cagA,vacA,iceA基因亚型进行检测.同时取胃窦、体、角黏膜各1块,经石蜡切片,HE染色,行组织病理学诊断.结果:GA组感染m2(43.1%)亚型菌株构成比最高,与GU(18.2%)和GC(17.9%)组相比差异有统计学意义(P=0.015,P=0.020),与GS(30.00%)组相比差异没有统计学意义(P= 0.084).在GA组中,感染s1m2亚型菌株有22例(44.9%),与本组病例中其他基因亚型相比差别均有统计学意义,与Gu、GC组感染s1m2亚型菌株相比差别有统计学意义(P=0.039),与GS组相比差别没有统计学意义(P=0.067).结论:中国辽宁地区人群感染s1m2型菌株与萎缩性胃炎发生有关.

AIM： To explore the relationship between Helicobacter pylori infection and associated gastric diseases. METHODS： Gastric biopsies were taken from 491 subjects, which were divided into supreficial gastritis （GS）, atrophic gastritis （GA）, gastric ulcer （GU） and gastric cancer （GC） groups, and 222 H pylori strains isolated from these patients were studied by PCR for differences in their genotypes cagA, vacA, and iceA. Three biopsies were taken for pathological diagnosis, from the gastric antrum, corpus and angularis, respec- tively. RESULTS： The H pylori rn2 infection rate in GA, GU and GC groups was 43.1%, 18.2% and 17.9%, respectively, and there was a statistical difference （P = 0.015 and P = 0.020）. Twenty-two slm2 subtype strains （44.9%） were found in GA group and there was a statistical difference compared with GU and GC groups （P = 0.039） but not with GS group （P = 0.067）. CONCLUSION： There is a positive relationship between infection with H pylori slm2 strain and GA.