Proteome Analysis of the Adaptation of a Phenol-Degrading Bacterium Acinetobacter sp. EDP3 to the Variation of Phenol Loadings

紧张 EDP3 从工业激活污泥被孤立。它根据 16S rRNA 基因序列与 97.0% 的身份属于 Proteobacteria 的鲸鱼群妈组到 Acinetobacter calcoaceticus。它能更长与大部分在房间温度容忍直到 1000mg .L-1 酚落后阶段。这显示更高的酚集中导致了生理的一些， genotypic 在细菌变化。因此，这研究的目的是在紧张 EDP3 调查这些回答到酚集中变化。Proteome 分析借助于二维的 polyacrylamide 胶化电气泳动(2D 页) 和 spectrometry (MALDI-TOF-MS ) 被进行在细菌内获得更深的卓见进适应回答的飞行质量的帮助矩阵的激光解吸附作用电离时间被进行。在 400mg .L-1 和 1000mg .L-1 酚种的紧张 EDP3 的 proteome 侧面的比较分析允许我们鉴别在在更高的酚集中下面起来调整的所有蛋白质之中，氧化压力蛋白质是主导的。热吃惊蛋白质的合成，在 GroEL 的 60000 陪伴，也被放大。另外，一膜蛋白质的表达式，腺苷 5'-triphosphate (ATP ) 绑定盒子(ABC ) 类型糖 transporter，被发现起来调整。腺苷 5'-triphosphate (ATP ) 和 RNA/protein 合成的抑制也被观察。

Strain EDP3 was isolated from an industrial-activated sludge. It belonged to the gamma group of Pro-teobacteria with an identity of 97.0% to Acinetobacter calcoaceticus according to the 16S rRNA gene sequences. It can tolerate up to 1000mg·L^-1 phenol at room temperature with a much longer lag phase. This indicates that higher phenol concentration has induced some physiological and genotypic changes in the bacterium. The aim of this study is, therefore, to investigate these responses to phenol concentration variations in strain EDP3. Proteome analysis is conducted by means of a two-dimensional polyacrylamide gel electrophoresis （2D PAGE） and matrix-assisted laser desorption ionization time of flight mass spectrometry （MALDI-TOF-MS） was conducted to obtain a deeper insight into the adaptive responses inside the bacterium. Comparative analysis of the proteome profiles of strain EDP3. grown in 400mg·L^-1 and 1000mg·L^-1 phenol allowed us to identify that among all the proteins up-regulated under the higher phenol concentration, oxidative stress proteins were dominant. The synthesis of a heat shock protein, 60000 chaperonin GroEL, was also amplified. In addition, the expression of one membrane protein, adenosine 5＇-triphosphate （ATP）-binding cassette （ABC） type sugar transporter, was found up-regulated. The inhibition of adenosine 5＇-triphosphate （ATP） and RNA/protein synthesis was also observed.