辣椒子叶离体培养及植株再生体系建立

Cotyledon Culture of Pepper in vitro and High Efficient System Establishment of Plant Regeneration

以10个辣椒品种的子叶为试材,探讨了影响辣椒子叶离体培养及再生的各种因素(子叶外植体的生理年龄、外植体的部位、基本培养基、激素、硝酸银及光照强度等),筛选出辣椒子叶高效芽分化培养基为MS+6-BA5.0 mg/L+IAA1.0 mg/L+AgNO34.0 mg/L,平均芽分化率达到90.5%;高效芽伸长培养基为MS+6-BA3.0 mg/L+IAA1.0 mg/L+GA32.0 mg/L+AgNO34.0 mg/L;生根培养基为1/2 MS+IAA 0.2 mg/L+NAA0.1 mg/L,建立了辣椒高效再生体系。

Ten varieties of pepper （Capsicum annuum L. ） were selected as experimental materials. The effects of seedling stage, genotypes, hormone, organic nutrient component and AgNO3 et al on the tissue culture of pepper cotyledon explants were investigated. As a result, the best media for in vitro regeneration of cotyledon explants of pepper were discovered as follows： the bud induction medium is MS＋6-BA 5. 0 mg/L＋IAA 1. 0 mg/L＋AgNO3 4. 0 mg/L; the bud elongation medium： MS＋6-BA 3. 0 mg/L＋IAA 1. 0 mg/L＋GAa2. 0 mg/L＋AgNOa 4. 0 mg/L;and the rooting medium： 1/2 MS＋IAA 0. 2 mg/L＋NAA 0. 1 mg/L Thereby a highly efficient regeneration system for pepper in vitro is established.