药用红树植物老鼠簕(Acanthus ilici folius)乙醇提取物的化学成分研究

Studies on chemical constituents in ethanolic extract from Acanthus ilicifolius as a pharmaceutic mangrove

目的探讨老鼠簕的化学成分。方法将老鼠簕全株粉碎后,用95%乙醇浸提3次,合并浓缩,浸膏的石油醚部位经反复硅胶柱层析分离,结合波谱学数据鉴定化合物结构。结果从石油醚部位分离得到8个化合物,分别为β-谷甾醇(Ⅰ)、豆甾醇(Ⅱ)、正十六烷酸(Ⅲ)、正二十八烷酸(Ⅳ)、4,22-二烯-3-酮豆甾烷(stig-masta-4,22-dien-3-one)(Ⅴ)、4-烯-3-酮豆甾烷(stigmast-4-en-3-one)(Ⅵ)、5,22-二烯-7-酮-3β-羟基豆甾烷(3β-hydroxystigmasta-5,22-dien-7-one)(Ⅶ)、2-唑啉酮(2-benzoxazolinone)(Ⅷ)。结论其中Ⅴ,Ⅵ,Ⅶ为首次从该植物中分离得到。

Objective To study the chemical constituents of Acanthus ilicifolius. Methods The air-dried and powdered plant material was extracted with 95 % ethanol. Silica gel and Sephadex LH-20 column chromotography were used in the isolation from the ethanol extracts of the whole plant, the compounds were determined on the basis of various modern spectroscopic analyses and physical constants. Results Eight compounds were isolated from the petroleum ether soluble portion, identified as β-sitosterol（ Ⅰ ）, stigmasterol （Ⅱ）, n-hexadecane acid （ Ⅲ ）, n-octacosanic acid（Ⅳ）, stigmasta-4,22-dien-3-one（ Ⅴ ）, stigmasta-4-en-a-one （Ⅵ）, 3β-hydroxystigmasta-5,22-dien-7-one （ Ⅶ ） and 2-benzoxazolinone （ Ⅷ ）. Conclusion Compound Ⅴ, Ⅵ, Ⅶ were obtained from the genus for the first time.