摘要
目的研究转人铜锌超氧化物歧化酶(hCu,Zn-SOD)突变基因聚球藻口服后的生物活性。方法给小鼠灌服转hCu,Zn-SOD突变基因聚球藻20d,然后测定小鼠谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活力以及丙二醛(MDA)含量。结果转hCu,Zn-SOD突变基因聚球藻可明显提高小鼠血清GSH-Px活力和全血血红蛋白CAT活性,显著提高小鼠血清和肝脏中Cu,Zn-SOD和总SOD(T-SOD)的活力,显著降低小鼠血清和肝脏的MDA含量。结论转hCu,Zn-SOD突变基因聚球藻有较强的抗氧化作用。
Objective To study the biological activities of Synechococcus sp. PCC7942 with trans-mutated hCu, Zn-SOD-gene. Methods Synechococcus sp. PCC7942 with trans-mutated hCu,Zn-SOD were administered orally for 20d to mice, then the activities of glutathione peroxidase （GSH-Px）, catalase （CAT）, superoxide dismutase （SOD） and the content of malondialdehyde （MDA） were determined. Results The activities of GSH-Px in serum and the activities of CAT in blood increased obviously; the activity of SOD in serum and liver increased markedly; the content of MDA in serum and liver decreased obviously. Conclusion Synechococcus sp. PCC7942 with trans-mutated hCu,Zn-SOD- gene had obvious antioxidant effect in vivo.
出处
《中国海洋药物》
CAS
CSCD
2007年第6期10-12,共3页
Chinese Journal of Marine Drugs
基金
福建省自然科学基金资助项目(C0510004)
