原发性肝癌中Tec的表达及其磷酸化水平

The expression of Tec and the level of its phosphorylation in primary hepatic carcinomas

目的用高通量的组织芯片技术，分析人正常肝组织、癌旁肝组织及肝癌组织中Tec的表达及磷酸化水平，探讨其表达差异的意义。方法用组织芯片技术结合免疫组织化学法检测肝癌组织、癌旁肝组织、正常肝组织中Tec的表达及其磷酸化水平。结果Tec在肝癌组织及非癌组织中阳性表达率差异无统计学意义，但在肝癌组织中的染色程度显著强于非癌组织，且与组织分级有关（P〈0．05）。Tec磷酸化水平在肝癌组织（73％）高于癌旁组织（42％）及正常肝组织（10％），P〈0．05。结论Tec的表达异常与肝癌的发生发展密切相关，抑制Tec的表达或直接降低其磷酸化水平可能影响肝癌的进程。

Objectives To detect the expressions of Tec tyrosine kinase in hepatocelluar carcinoma and the levels of phosphorylation of tyrosine kinase in liver cancer tissues, paracancerous tissues and normal liver tissues and to find the significance of their differences. Methods 200 specimens of tissues, including liver cancer tissues, surrounding liver tissues not more than 1.5 cm from the cancers, and normal liver tissues were investigated for Tec protein expression and Tec phosphoralaton by tissue microarrays and immunohistochemistry （SP method）. Results The positive immunohistochemical stainings of Tec in cancerous tissues and non-cancerous tissues showed no obvious differences, nevertheless, the immunostaining levels in liver cancer tissues were much higher than in non-cancerous tissues and they correlated with the grading of tumors （P 〈 0.05）. The phosphorylation of Tec was significantly expressed in liver cancer tissues （73%） in comparison with other tissues （42%, 10% both P 〈 0.05）, but it did not correlate with any clinicopathological characteristics. Conclusion Overexpression of Tec is associated with the tumorigenesis and development of liver.cancer; inhibiting Tec or degrading Tec phosphorylation directly might affect the progression of liver cancer.