摘要
目的观察齐墩果酸(Oleanolic Acid,OA)对慢性粒细胞白血病系K562细胞中血管内皮生长因子(VEGF)表达的影响。方法采用MTT实验观察OA对体外培养K562细胞增殖抑制作用,用RT-PCR以及Western印迹法研究OA处理后K562细胞中VEGF基因表达的变化。并用ELISA方法检测培养液中VEGF的表达。结果OA能抑制K562细胞生长,呈一定的量效特征;并能抑制K562细胞中VEGF的表达。结论OA通过下调VEGF表达,抑制白血病细胞的增殖。
Objective To observe the effect of oleanolic acid on expression of vascular endothelial growth factor （VEGF） in human leukaemia cancer cell line K562. Methods The inhibitory effect of oleanolic acid on viability of K562 cells was assessed by MTT assay. The expression of VEGF mRNA and protein were determined by RT-PCR and Western blot, respectively. The expression of VEGF in culture medium was detected by ELISA. Results Oleanolic acid inhibited growth of K562 ceils in a dose-dependent manner and down-regulated expression of VEGF. Conclusions The anti-tumor mechanism of oleanolic acid is related with inhibition of VEGF expression, which causes down-regulation of VEGF, therefore decreases tumor growth.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2007年第24期2371-2373,共3页
Chinese Journal of Gerontology
基金
黑龙江省自然科学基金资助项目(D200670)
黑龙江省卫生厅重点科研项目(2006334)
佳木斯大学科研项目(S200603)
关键词
白血病
齐墩果酸
VEGF
K562细胞
Leukaemia cancer
Oleanolic acid
Vascular endothelial growth factor （VEGF）
K562 cell
