降钙素对小鼠牙齿萌出途中破骨细胞的影响

Effects of calcitonin on osteoclast during tooth eruption in mouse

目的:在体研究降钙素对正常小鼠牙齿萌出过程中破骨细胞的影响,并分析其机制。方法:同一窝10只出生3d小鼠随机分成对照组和降钙素组,后者皮内注射鲑鱼降钙素每日1U/100g,连续3d。自注药起7d取其下颌骨,分别用酶组织化学和免疫组织化学的方法检测破骨细胞、RANKL阳性细胞在牙胚周围组织中的变化情况。结果:降钙素组的平均破骨细胞数、RANKL表达较对照组明显降低。结论:降钙素对正常小鼠牙齿萌出过程中的破骨细胞可能有抑制作用,这可为牙齿萌出的研究提供一定参考。

AIM：To observe calcitonin on osteoclast during tooth eruption in mouse and analyze the mechanism.METHODS：Ten brood pups at 3d after birth were devided randomly to control and calcitonin groups.The mouses in calcitonin group were injected with calcitonin for 3days（1U /100g/day）.7days after injection the pups were sacrificed and mandible were taked out.The number of the osteoclasts was detected with tartrate-resistance acid phosphatase（TRAP）staining.Immunohistochemistry was used to examine the expressions of RANKL.RESULTS：The number of osteoclasts in calcitonin group was less than that in the control group（P〈0.05）.There was significant difference for RANKL proteinum between calcitonin and the control groups.CONCLUSION：Calcitonin could inhibit osteoclast during tooth eruption in mouse,which may provide reference to investigation about tooth eruption.