多西环素牙周缓释凝胶的配方筛选研究

Formula Screening Study on Periodontal Sustained-release Gel with Doxycycline

目的探索一种牙周缓释凝胶的最佳配方。方法将聚乳酸-乙醇酸共聚物(PLGA)浓度、溶剂配比和多西环素浓度按三个水平作正交设计,进行体外释放度试验,以第6天的释放百分率和凝结时间的综合分为指标,计算极差。结果按极差大小,PLGA浓度>溶剂配比>多西环素浓度,最佳配方为PLGA浓度15%,1MP:GTA=7:3,多西环素浓度为6%。结论用正交设计进行配方筛选是一种较好的试验方法。

OBJECTIVE To explore a best formula of sustained-released gel. METHODS Made an orthogonal design at 3 levels of 3 factors （ concentration of PLGA, portion of solvent and concentration of doxycycline） to measure the concentration of doxycycline in vitro, caculated the range using the releasing rate on the sixth day and score of coagulate time as parameter. RESULTS According to the range, PLGA＇s concentration 〉 solvent＇s portion 〉 doxycycline＇s concentration,the best formula was：the concentration of PLGA80 ： 20 = 15 % （w/w） , 1MP： GTA = 7：3 and doxycycline concentration = 6 % （w/w）. CONCLUSION It is a good screening method to find out the best formula by orthogonal design.