苦参碱对博来霉素所致肺间质纤维化大鼠的保护作用

Protecting effect of matrine on pulmonary interstitial fibrosis in rats

目的:研究苦参碱对肺间质纤维化大鼠的保护作用。方法:采用气管内灌注博来霉素(BLM)的方法建立大鼠肺纤维化模型,设正常对照组、模型组、小剂量苦参碱组(50 mg/kg)、大剂量苦参碱组(100 mg/kg)和氢化可的松组(100 mg/kg),每组18只,再各分为d 7、14和d 28组。观察各组不同时期病理切片和肺组织羟脯氨酸(HYP)浓度,测定肺组织丙二醛(MDA)和谷胱甘肽过氧化物酶(GSH- PX)含量。结果:苦参碱组和氢化可的松组的肺泡炎和肺纤维化程度及HYP含量显著地低于模型组(P<0.05);苦参碱和氢化可的松能明显降低肺间质纤维化大鼠肺组织MDA水平,提高GSH-PX含量(P<0.01)。结论:苦参碱能减轻BLM诱导的大鼠肺纤维化,这种作用有可能通过改善肺间质纤维化大鼠体内氧化应激状态实现。

AIM： To study the effect of matrine on bleomycin-induced pulmonary fibrosis in rats and its mechanism. METHODS： Pulmonary fibrosis model was estalished in male Wistar rats by intratracheal instillation of bleomycin（BLM）. Then the rats were given matrine by the intraperitoneal injection. Histological changes of the lungs were evaluated by HE stain and Masson＇s trichrome stain. Collagen content of the lung tissue was assessed by hydroxyproline concentration. Malondialdehyde （MDA） and glutathione peroxidase （GSH-PX）of the lung tissue were measured by colorimetric method. RESULTS： Pulmonary fibrosis of matrine group （OM） and hydrocortisone group （P） was improved as compared with that of group BLM. Hydroxyproline concentrations of group OM and group P were lower than that of group BLM （ P 〈 0.05）; The levels of MDA in the lung tissue were significantly increased in group OM and group P as compared with group BLM（ P 〈 0.01）. Therapeutic efficacy of the matrine was the same as the treatment of hormone in the rat model of bleomycin-induced pulmonary fibrosis. CONCLUSION： Matrine alleviates bleomycin-induced pulmonary fibrosis in rats, which related to the effectively inhibition of oxidative stress in lung tissues.