野生毛葡萄皮中原花青素提取工艺研究

Study on extracting procedure of proanthocyandins from wild grape skin

以野生毛葡萄皮为原料,以原花青素提取率考察指标,研究提取溶剂种类、浓度、料液比、提取温度、提取时间、提取次数等因素对原花青素提取效果的影响。通过正交实验,确定野生毛葡萄皮中原花青素的最佳提取工艺条件为:提取溶剂为60%乙醇,料液比1∶9,提取温度60℃,提取时间2.5 h,提取3次。在此优化条件下野生毛葡萄皮中原花青素的平均提取率为5.02%.

The content of proanthocyandins from wild grape skin was regarded as the index of examination. It was studied the impact of the extracting solvent concentration, emperature, solid-liquid ratio, extracting time and extracting times on the extraction. According to the orthogonal experimental results, the optimum extracting conditions of proanthocyandins from wild grape skin were showed as follows： 60 % ethanol as extracting .solvent, 1：9 for the solid-liquid ratio, 60℃ for the extracting temperature, 2.5h for the extracting time and extracting 3 times. At optimal conditions for experimental repeatability, the average extraction rate of proanthocyandins from wild grape skin is 5.02 %.