摘要
采用CTAB法提取了人参(Panax ginseng)根基因组DNA,根据植物叶绿体16S rDNA和线粒体18SrDNA与细菌16S rDNA序列具有高度同源性,用扩增细菌16S rDNA的一对通用引物(8f,1492r)扩增了人参细胞器核糖体小亚单位DNA。对扩增产物进行了克隆与测序,经多序列比对,扩增片段分别与已知植物叶绿体16S rDNA和线粒体18S rDNA具高度同源性,表明该对引物可以用来扩增绝大多数植物细胞器核糖体小亚单位DNA,可以作为鉴定植物叶绿体16S rDNA和线粒体18S rDNA的一种基本实验技术。
The total DNA was extracted from roots of Parax ginseng. According to the sequence homology between bacterial 16S rDNA and small sub-unit ribosome DNA （SSU rDNA）in plant organelles,a pair of bacterial 16S rDNA primers （8f, 1492r）was selected to amplify 16S rDNA in plastid and 18S rDNA in mitochondria of Panax ginseng directly. The PCK products about 1.5kb and 1.9kb were purified and cloned to T-easy vector separately,and the segments were sequenced. The results showed that 1.5kb sequence had significant homology with chloroplast 16S rDNA and 1.9kb with mitochondria 18S rDNA in plant organelles. It could be concluded that the pair of primers and method could be used to amplify SSU rDNA in organdies of plants.
出处
《生物技术通报》
CAS
CSCD
2008年第1期124-127,共4页
Biotechnology Bulletin
基金
国家自然科学基金项目(编号:30370032,30770069)
教育部高等学校博士学科点专项科研基金项目(编号:20060028001)
