辣椒根腐病拮抗细菌的筛选及其生物学特性研究

Screening and Study on Biological Characteristics of Antagonistic Bacteria Against Fusarium solani

以辣椒根腐病原菌(Fusarium solani)为指示菌,从贵州辣椒根际筛选到5株具有明显拮抗效果的细菌,编号分别为:SC2、SC2-4、SC2-4-1、SC2-4-2、SC2-4X。上述菌株抗菌谱较广,对黄瓜枯萎病原菌(Fusarium oxysporum f.sp.cucumerinum)、黄瓜霜霉病原菌(Pseudoperonospora cubensis)、芹菜灰霉病原菌(Botrytis cinerea Pers)以及西红柿灰霉病原菌(Botrytis cinerea)也具有良好的拮抗性能。经形态学测定、生理生化分析和16S rDNA序列分析,将SC2、SC2-4-2、SC2-4X鉴定为多粘类芽孢杆菌(Paenibacillus polymyxa),SC2-4、SC2-4-1鉴定为枯草芽孢杆菌(Bacillus subtilis)。SC2菌株产生的抗菌物质可能为蛋白类或抗菌肽类物质。

With Fusarium solani as indicator,5 antagonistic bacteria were screened from the rhizosphere of capsicum in Guizhou and were named as SC2,SC2-4,SC2-4-1,SC2-4-2,and SC2-4X,respecfively, They have a broad-spectrum antimicrobial activity and can antagonize Fusarium oxysporum f. sp. cucumerinum,Pseudoperonospora cubensis,Botrytis cinerea Pers and Botrytis cinerea. Isolates SC2,SC2-4-2,and SC2-4X were identified as Paenibacillus polymyxa and isolates SC2-4 and SC2-4-1 were identified as Bacillus subtilis by morphological test,physiological and biochemical tests and 16S rDNA sequence analysis. The isolate SC2 probably produce antimicrobial protein or peptides.