大鼠胰岛与生长因子共微囊提高囊内胰岛存活率

Growth factor coencapsulated with rat islets improves survival of encapsulated islets

目的探讨微胶囊胰岛皮下移植治疗化学性糖尿病小鼠和生长因子(growth factor,GF)与大鼠胰岛共微囊对提高囊内胰岛存活率的作用。方法选取C57BL/6雄性小鼠皮下预血管化糖尿病模型25只,行微胶囊大鼠胰岛皮下移植。随机分为5组,每组5只。①A组:GF与大鼠胰岛共微囊移植组;②B组:微胶囊大鼠胰岛移植组;③C组:GF微囊移植组;④D组:大鼠胰岛移植组;⑤E组:空囊移植组。观察受体小鼠的血糖和体重变化,移植后8周比较微胶囊内胰岛存活数。结果移植后1周至移植后3周A组与B组间血糖均有非常显著差异(P<0.01),移植后4周至移植后8周A组与B组间血糖无显著差异(P>0.05),移植后1周至移植后4周B组与C组间血糖均有非常显著差异(P<0.01)。C组血糖异常升高,移植4周后小鼠陆续死亡。D组和E组移植无效。A组和B组间各实验点小鼠体重均无显著差异(P>0.05),A组小鼠体重成模时下降,移植2周后开始上升。A组和B组胰岛存活数有非常显著差异(F=36.84,P<0.01)。结论GF与大鼠胰岛共微胶囊移植,GF可促进胰岛的细胞增殖和再生,显著提高囊内胰岛的存活率。

Objective To see whether prevascularized subcutaneous transplantation of SD rat islets coencapsulated with growth factor （GF） normalizes blood glucose of STZ-induced diabetic mice and improves survival of encapsulated SD rats islets. Methods Twenty five subcutaneously prevascularized streptozocin（STZ）-induced diabetic mice using microencapsulated islets for transplantation were equally randomized to 5 groups： Group A, using SD rat islets coencapsulated with GF for prevascularized subcutaneous transplantation; Group B, using encapsulated rat islets for prevascularization subcutaneous transplantation; Group C, D and E, serving as controls. Post-transplantation blood glucose and body weight of STZ-induced diabetic mice were observed. The number of surviving rat islets was counted 8 weeks after transplantation. Results The mean blood glucose level of Group A and B was normalized 7 days after transplantation. Xenotransplantation reversed the loss of body weight and led to a continuous increase in body weight, the difference between the two groups being insignificant. DTZ-positive microencapsules were counted 8 weeks after transplantation. More DTZ-positive micro- encapsules were found in Group A （F = 36.84, P%0.01）. Conclusions Rat islets coencapsulated with GF prevascularization subcutaneous transplantation is an effective strategy for promoting proliferation and regeneration of islets and improves survival of encapsulated islets of diabetic animals.