PP2药物抑制乳腺癌细胞侵袭与增殖的机制

Effect of Proliferation and Invasion of Breast Cancer Cell by PP2

目的以PP2[4-Amino-5-(4-Chloro-Phenyl)-7-(t-Butyl)Pyrazolo[3,4-d]Pyri midine]药物(src激酶抑制剂)处理乳腺癌细胞,检测src激酶及E-cadherin蛋白表达水平的变化,观察PP2对乳腺癌细胞增殖和侵袭能力的影响,并探讨其可能的机制。方法PP2处理乳腺癌细胞MDA-MB-231,Western blot检测src及其相关蛋白的表达;Boyden小室实验检测细胞侵袭能力;MTT检测细胞的增殖能力。结果PP2处理MDA-MB-231细胞后,src表达水平明显降低,E-cadherin表达水平升高;细胞的增殖和侵袭能力均受到明显抑制,剂量越高抑制作用越明显(P<0.05)。结论PP2通过提高细胞粘附分子E-cadherin的表达,从而抑制乳腺癌细胞MDA-MB-231的增殖和侵袭能力。

Objective To study whether PP2 [4-Amino-5-（4-Chloro-Phenyl）-7-（t-Butyl） Pyrazolo [3,4-d] Pyrimidine] could change the expression of src kinase and E-cadherin protein, and detect the proliferation and invasion of breast cancer cell. Methods After treating MDA-MD231 with PP2, western blot was performed to determine the expression of src and E-cadherin; The proliferation was been tested by MTT assay; Boyden chamber assay was used to examine the effect of the invasion of MDA-MD231 cell. Results Western blot show if the dosage of PP2 increase, the expression of src is down-regulated and E-cadherin is up-regulated. MTT assay show that the proliferation of MDA-MPr231 have association with PP2 dos-age （P〈 0.05）. Boyden chamber assay show that the invasion is down- regulated when the dosage of PP2 is enhanced （ P 〈 0. 05 ）. Conclusion PP2 can strongly inhibit src and enhance the expression of the downstream protein of E-cadherin. The proliferation and invasion of MDA-MB-231 cells is also inhibited by PP2.