蛋白激酶C-α、CyclinD1和Cdk4在大肠癌中表达的相关性及临床意义

Expression of Protein Kinase C alpha,CyclinD1 and Cdk4 Proteins in Colorectal Carcinomas and Their Clinical Significance

目的探讨PKC-α,CyclinD1和Cdk4在大肠癌中表达的相关性及其与临床病理学特征的关系。方法应用免疫组化SP法对83例大肠组织中PKC-α,CyclinD1和Cdk4的表达进行检测。结果PKC-α在大肠癌组织中阳性表达31/47(66.0%)与正常组织3/15(20.0%)及腺瘤10/21(47.6%)相比差异有统计学意义(P<0.05),与大肠癌分化程度,Dukes分期,淋巴结转移及CEA水平明显相关(P<0.05)。Cy-clinD1和Cdk4在大肠癌中阳性表达分别为26/47(55.5%)、28/47(59.6%),与正常组织中2/15(13.3%)、1/15(6.70%)及腺瘤组织中8/21(38.1%)、9/21(42.9%)相比呈递增趋势(P<0.05)。大肠癌中CyclinD1阳性表达与肿瘤分化程度,Dukes分期,淋巴结转移呈正相关(P<0.05),而与CEA水平关系不大(P>0.05);Cdk4阳性表达则与分化程度,Dukes分期有关(P<0.05),与淋巴结转移及CEA水平关系不密切(P>0.05)。PKC-α与CyclinD1呈正相关关系(r=0.348,P<0.05),CyclinD1和Cdk4呈正相关关系(r=0.393,P<0.05),PKC-α和Cdk4则无明显相关性(r=0.167,P>0.05)。结论蛋白激酶C-α与CyclinD1及Cdk4的过表达在大肠癌的发生、发展中起着重要的协同作用。三者的过表达与大肠癌的分化程度,Dukes分期密切相关。

Objective To investigate the relationship between the expression of PKC-α,CyclinD1 and Cdk4 and the clinical significance in colorectal carcinomas. Methods Immunohistochemical staining was used to detect the expressions of PKC-α,CyclinD1 and Cdk4 in normal colorectal tissues, adenoma and carcinoma, respectively. Results The Positive rates of PKC-α expression in normal colorectal tissues, adenoma and carcinomas were 20%, 47%, 66. 0%, respectively （P〈0. 05）, associated with the tumor cellular differentiation ,Dukes phase and lymph node metastasis and CEA, respectively （P 〈0. 05 ）. Overexpression of CyclinD1 and Cdk4 were 55.3 %, 59. 6 % in colorectal carcinomas, showed an increasing tendency in colorectal carcinogenesis, respectively （P〈0. 05）; the expression of CyclinD1 was correlated closely with differentiated degree, Dukes phage, lymph node metastasis and CEA, respectively （P〈0. 05） ;the expression of Cdk4 was associated with differentiated degree, Dukes phase and CEA, respectively （P〈0. 05）. There is a positive relationship between PKC-α and CyclinD1 （r= 0. 348,P〈0. 05）, There is a positive relationship between CyclinD1 and Cdk4 （r= 0. 393,P 〈0. 05）. Conclusion Overexpression of PKC-α, CyclinD1 and Cdk4 may contribute to the pathogenesis of colorectal carcinoma, and it was correlated closely with differentiated degree, Dukes phase and CEA.