人体胆囊结石难溶物的组成及在其胆石形成过程中的作用

Study on the Ingredient and Its Function of the Insoluble Human Gallbladder Stone Remainder

目的:明确人体胆囊结石难溶物的基本组分并探讨其在胆囊结石形成与生长过程中的作用。方法:人体胆囊结石经氯仿、乙醇、乙醚、盐酸等溶剂溶解后取得其难溶剩余物,用傅里叶变换红外光谱仪对其主要成分进行分析研究。结果:人体胆囊结石难溶物主要由胆红素钙盐、蛋白质、脂肪酸钙盐、磷酸钙盐、碳酸钙等组成。结论:蛋白质及其复合物在胆石中形成网架结构并成为促使胆固醇晶体结晶生长和其他非晶物质沉积的基质,而钙等阳离子与磷脂等双极性分子的结合削弱了双极性分子对胆固醇的溶解作用导致了胆固醇结晶和沉积的加速。

Objective： To make sure the basic ingredient of the Insoluble Human Gallbladder Stone Remainder and discuss its function in creating and forming the Human Gallbladder Stone. Methods： The Insoluble Human Gallbladder Stone which were dissolved with chloroform,ethanol,ether and hydrochloric acid were analysed by Fourier transform infrared spectrum. Results： It was find that bilirubinate calcium salt,proteins,fatty acid calcium salt,phosphoric acid calcium salt, calcium carbonate are the main components in the insoluble remainder of gallbladder stone. Conclusion： The protein and its compounds make the role of reticular structure to promote deposition of cholesterol crystals and other noncrystalline ,but the combination between positive ions and bipolarity molecules such as calcium and phosphatide has weakened bipolarity molecule dissolution to cholesterol. It leds to the acceleration of cholesterol crystals and deposition.