葡萄糖代神经氨酸体外抑制钙调蛋白活性

Inhibition of Glucolylneuraminic Acid Analogue on the Calmodulin Activity in Vitro

三种唾液酸亚型５－乙酰神经氨酸（５ＡｃＮｅｕ）、５，９－二乙酰神经氨酸（５，９ＡｃＮｅｕ）和葡萄糖代神经氨酸（ＮｅｕＧｃ）皆从小鼠肿瘤腹水中通过阴离子交换树脂法获得．葡萄糖代神经氨酸（２μｇ／ｍｌ）体外显示抗钙调蛋白活性，另两种亚型在１０μｇ／ｍｌ浓度下仍不显示该生物活性．

umor malignancy and metastasis can bring about the increment of synthesis of sialic acids, especially glucolylneuraminic acids analogues (NeuGc). In this work, it is found that NeuGc(2μg/ml) can inhibit the calmodulin activated Ca++Mg++ ATPase activity (inhibitory rate was 20%), whereas the 5AcNeu or 5,9AcNeu at the concentration of 10 μg/ml shows no response. This shows that NeuGc might play a feed back role in tumors.