东方百合LsCCR1基因的克隆及表达分析

Cloning and Expression Analysis of the LsCCR1 Gene from Lilium Oriental Hybrids

百合茎秆的挺度直接关系到百合花采收后的货架寿命等重要商品性状,而这些商品性状与植物茎秆中的木质素的含量高低有关。本文应用RLM-RACE技术,从东方百合索蚌植株中克隆了木质素合成相关基因--肉桂酰辅酶A还原酶,定名为LsCCR1。该基因cDNA全长为1499bp,包括完整的阅读框,编码388个氨基酸。多重比对分析发现百合LsCCR1基因与其他植物上已经发现的CCR基因同源性多介于55%￣66%之间。利用半定量PCR检测LsCCR1基因在百合不同组织中的表达差异,结果显示LsCCR1基因主要在百合的茎秆中表达,根部次之,叶片、鳞茎及花蕾中表达量较少,其表达模式与拟南芥、桉树及大麦中CCR基因的表达模式类似。

The post-harvest lifetime of lily follower is one of the important marketing characters. And these traits were mostly related with the lignin contents in the stem. In present paper Cinnamoyl-CoA reductase （CCR） gene, which was one of the key genes in the pathway of the lignin, had been cloned from Lilium oriental hybrids （Sorbonne） plants by RLM-RACE. The LsCCR1 is a 1 499 bp full-length eDNA with whole ORF encoding 388 amino acids. The multiple comparison results of protein sequences shown that, the homology of CCR between lily and other plants is not very high, mostly in and between 55%-66%. The expression of LsCCR1 in different tissues of lily was detected by semi-quantitative RT-PCR, which mainly expressed in stem and root, and at low level in leaf, bulbscale and flower bud, the expression pattern would be much more similar to that of the genes, A tCCR, EuCCR and HvCCR.