156株洋葱伯克霍尔德菌的临床分布及耐药性分析

The source and antibiotic resistance of 156 strains of Burkholderia cepacia

目的了解2003--2006年我院临床分离的洋葱伯克霍尔德菌的标本来源、临床分布和耐药性。方法用MicroScanWalkAway-4-0全自动微生物鉴定和药敏系统对我院临床分离的洋葱伯克霍尔德菌进行鉴定和药敏试验，并分析结果。结果20032006年共分离出156株洋葱伯克霍尔德菌，痰标本中分离菌株数占84．0％（131／156），血液中占5．1％（8/156）。药敏试验结果显示，多数测试的抗菌药物耐药率均大于50％；耐药率小于50％有哌拉西林他唑巴坦（4．8％～21．4％）、复方磺胺甲嗯唑（5．6％～23．8％）和头孢他啶（17．2％～40．0％）。结论洋葱伯克霍尔德菌对多数抗菌药物具有较高的耐药性，临床抗感染治疗应以分离菌株的体外抗菌药物敏感性为依据。

Objective To investigate the source and antibiotic resistance of Burkholderia cepacia isolated from 2003 to 2006. Methods A total of 156 B. cepacia strains were isolated from West China Hospital, Sichuan University. Bacterial identification and antibiotic susceptibility testing were carried out using MicroScan WalkAway 40 system. Results From 2003 to 2006, 156 strains of B. cepacia were isolated. Sputum was the main source of B. cepacia （84.0%）, followed by blood （5.1%）. Most B. cepacia isolates （〉50%） showed resistance to most of the antibiotics tested. However, less than 50% of the isolates were resistant to piperacillin-tazobactam （4.8% -21.4% ）, trimethoprim-sulfamethoxazole （ 5.6% -23.8% ） or ceftazidime （ 17.2 %- 40.0%）. Conclusions B. cepacia is highly resistant 1o most antibiotics. Treatmenl of B. cepacia infection should be based on the results of susceptibility test.