膀胱移行细胞癌中人类端粒酶逆转录酶的表达及意义

Expression and Significance of Human Telomerase Reverse Transcriptase in Bladder Neoplasm

目的检测人类端粒酶逆转录醇（hTERT）在膀胱肿瘤中的定量表达，探讨其与膀胱肿瘤生物学行为的关系及在膀胱肿瘤中表达的价值与临床意义。方法采用免疫组织化学法和高清晰彩色医学图文分析系统，对45例膀胱移行细胞癌（BTCC）中的hTERT表达进行定量检测，并用13例正常膀胱组织标本作为对照。结果BTCC组织标本中均可见有hTERT的表达，正常膀胱组织中未见表达，表达强度随病理分级、临床分期的增高以及肿瘤的复发而相应增高，差异具有统计学意义（P〈0．05）。结论hTERT在膀胱肿瘤中表达上调，可以作为诊断、监测复发的指标。

Objective To observe the clinical significance of quantitative expression of human telomerase reverse transcriptase （hTERT） in bladder neoplasm, and the correlation between hTERT and clinico - biological behavior of bladder neoplasm. Methods The quantitative expression of hTERT was detected in 45 cases of bladder transitional cell （BTCC） carcinoma tissues （tumor group） and 13 cases of human normal bladder mucosa tissues （control group）, with immunohistochemistry method and HMIAS- 2000 image analysis system. Results The expression of hTERT in tumor group is positive and negative in control group. The quantitaty of hTERT increased with pathologic grade, clinical stage and the tumor recurrence （ P 〈 0.05 ）. Conclusion The up - regulated expression of hTERT in BTCC suggests that it could be a marker for the diagnosis and the recurrence of BTCC.