骨碱性磷酸酶在小儿佝偻病早期诊断中的应用

Value of bone alkaline phosphatase detection in early diagnosis of rickets of children

目的研究骨碱性磷酸酶测定在小儿佝偻病诊断中的应用,为佝偻病的筛查及早期诊断提供依据。方法由儿童保健医生按照"婴幼儿佝偻病防治方案"诊断标准将上述儿童分为佝偻病组(599例)和正常组(787例)。应用骨碱性磷酸酶试剂盒对上述1 386例儿童进行血浆骨碱性磷酸酶含量测定,同时检测血清钙含量。结果全部受检儿童骨碱性磷酸酶活性升高者927例,正常459例,其中佝偻病组骨碱性磷酸酶活性升高者578例,检出率为96.49%(578/599),正常组骨碱性磷酸酶升高者349例,检出率为44.35%(349/787),两组差异有统计学意义(2χ=417.60,P<0.01)。血骨碱性磷酸酶阳性率与血钙阳性率比较,2χ=69.95,P<0.01,有统计学意义。结论通过血骨碱性磷酸酶和血钙检测及比较分析证明骨碱性磷酸酶在小儿佝偻病早期筛查及诊断中具有重要意义。

Objective To investigate value of bone alkaline phosphatase （BAPA） detection in early diagnosis of rickets of children so as to provide a basis for screening and early diagnosis of the disease. Methods The serum BAPA level of 1386 children was detected with BAPA Agent Kit, meanwhile, the serum calcium content of all children was detected. Results All the children were divided into rickets children group （ n = 599 ） and normal children group （ n = 787 ） （ diagnosed by child health physicians according to diagnostic criteria worked out by Infantile Rickets Prevention and Treatment Program）. Totally, the activity of BAPA of 927 children was increased and that of 459 children was normal. Of which, 578 cases of increased activity of BAPA were seen in the rickets children group and the detectable rate was 96. 49% , other 349 cases of increased activity of BAPA were seen in the control group and the detectable rate was 44.35%. There was a significant difference between the two groups （x^2 = 417.60, P 〈 0.01 ）. In rickets positive detectable rate, there was a significant difference between serum BAPA detection and serum calcium detection （x^2 = 69.95, P 〈 0.01 ）. Conclusion As compared with serum calcium detection, serum BAPA detection is of more important significance in early screening and diagnosis of rickets of children.