釉基质蛋白对人角质形成细胞粘附、增殖及迁移影响的体外研究

Effects of enamel matrix proteins on the attachment、proliferation and migaration of human keratinocytes in vitro

目的:观察釉基质蛋白对体外培养的人角质形成细胞粘附、增殖及迁移等生物功能的影响。方法:消化法培养人正常角质形成细胞,采用MTT比色法及体外划痕法,观察人正常角质形成细胞在不同浓度釉基质蛋白(50、100、150及200μg/ml等浓度的EMPs)包被的培养孔表面粘附、增殖及迁移情况,试验各组依次命名为EP1、EP2、EP3、EP4组。结果:①细胞粘附性实验中,EP2、EP3及EP4组在接种细胞4.5h后结果均高于空白对照组(P<0.05);②细胞增殖实验中,在各时间点各组之间无统计学差异(P>00.05);③细胞迁移实验中,各实验组和空白对照组间并无统计学差异(P>0.05)。结论:EMP对人正常角质形成细胞粘附有促进作用,而对其增殖和迁移则没有影响。

Objective The purpose of this study is to investigate the influence of enamel matrix proteins （EMPs） on the attachment ,proliferation and migaration of cultured human keratinocytes. Methods The third passage keratinocytes were exposed to various concentrations of EMPs （50,100 ,150and 200μg/ml which was named group EP1,EP2,EP3, EP4）.Controls were human keratinocytes cultured in SFM medium without EMPs. The cell attachment and proliferation rates was measured by MTT method. The cell migration was measured by in vitro wound-healing assay. Results ① There were significant differences between the control group and experimental group EP2,EP3 and EP4 in the cell attachment experiment. ②There were no significant differences between the control group and experimental group in the cell proliferation experiment. ③There were no significant differences between the control group and experimental group in the in vitro wound-healing assay. Conclusion The results demonstrate that EMPs stimulate human keratinocytes attachment but have no effect on keratinocytes proliferation and migaration.