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硝苯地平对牙周膜重建中基质金属蛋白酶的影响 被引量：2

The effect on matrix metalloproteinase of Nifedipine during peridontal membrane remodeling
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摘要 目的:研究Ca2+通道阻滞剂-硝苯地平在正畸牙周膜重建过程中对基质金属蛋白酶(matrix metalloproteinase,MMP)表达与定位的影响。方法:选取60只雄性SD大鼠随机分为加力组、加力+低浓度硝苯地平组和加力+高浓度硝苯地平组,每组再按观察时间点的不同分为5组。用免疫组化方法结合计算机光密度分析系统检测并比较各处理组样本的张力侧与压力侧MMP-13和MMP-10的表达。结果:硝苯地平可以引起正畸牙周膜中基质金属蛋白酶表达的减少,并呈药物浓度增加而减少的更加明显;牙齿受力两侧比较,压力侧变化更显著。结论:硝苯地平阻断Ca2+通道可以使正畸牙周膜中的基质金属蛋白酶表达减少和定位差异减小,即牙周膜成纤维细胞不再对外力产生特异性反应。 Objective To study the effect on expression and localization of matrix metalloproteinase after using the calcium ion channel blocker-Nifedipine during periodontal membrane remodeling. Methods The expression of MMP-13 and MMP-10 on tension side and pressure side were examined by immunehistochemical dyeing and analyzed-with photodensitometry according to different groups. Results The expression of MMP decreased after using Nifedipine,and the decline was more obviously with high concentration of Nifedipine. The impact to pressure side was more significantly. Conclusion Blocking the calcium ion channel by Nifedipine can decrease the MMP expression and diminish the locating difference. Fibroblasts of periodontal membrane can not generate specific reaction to the force.
作者 冯冬菲 张苗苗 唐林
机构地区 哈尔滨医科大学附属口腔医学院口腔正畸科
出处 《中国美容医学》 CAS 2008年第1期80-83,共4页 Chinese Journal of Aesthetic Medicine
基金 省博士创新基金(XJSCX2007-0328HLJ)
关键词 硝苯地平 牙周膜重建 基质金属蛋白酶 钙离子通道 nifedipine periodontal membrane remodeling matrix metalloproteinase calcium ion channel
分类号 R783 [医药卫生—口腔医学]
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参考文献10

  • 1彭庭莉,周继祥.应力作用下基质金属蛋白酶在牙周组织改建中的作用[J].华南国防医学杂志,2005,19(5):44-46. 被引量：3
  • 2Arora PD,Bibby KJ, McCulloch CAG. Slow oscillation of free intracellular calcium ion concentration in human fibroblast responding to mechanical stretch [J].J Cell Physiol, 1994,161 (2): 187-200.
  • 3Waddington RJ,Embery G. Proteoglycans and orthodontic tooth Movement[J].J Orthod, 2001, 28 (4):281-290.
  • 4Schonherr E, Hausser HJ. Extracellular matrix and cytokines: a functional unit[J] .Dev Immunol,2000,7 (2-4): 89-101.
  • 5Krishnan V, Davidovitch Z, Cellular, molecular, and tissue-level reactions to orthodontic force [J],Am J Orthod Dentofacial Orthop, 2006,129 (4):469,e 1-32.
  • 6Kataoka M, Shimizu Y, Kunikiyo K. Nifedipine induces gingival overgrowth in rats through a reduction in collagen phagocytosis by gingival fibroblasts[J].J Periodontol,2001,72(8): 1078-1083.
  • 7KS Ko,PD Arora,V Bhide,et al, Cell-cell adhesion in human fibroblasts requires calcium signaling[J].J Cell Science,2001,114(6): 1155-1167.
  • 8孙兴旺,于国华.成纤维细胞钙离子通道与纤维化的研究进展[J].医学综述,2005,11(4):295-297. 被引量：2
  • 9Takahashi I,Nishimum M,Onodera K.Expression of MMP-8 and MMP-13 genes in the periodontal ligament during tooth movement in rats[J].J Dent Res, 2003, 82(8):646-51.
  • 10Leonardi R, Talic NF,Loreto C,MMP-13 (collagenase 3) immunolocalisation during initial orthodontic tooth movement in rats[J].Acta Histochem,2007,109(3):215-220.

二级参考文献17

  • 1Cho MR, Marler JP, Thatte HS, et al. Control If calcium entry in human fibroblasts by frequency-dependent electrical stimulation [J]. Front Biosci,2002,7(1) :1-8.
  • 2Eskes R, Antonsson B, Osen-sand A, et al. Bax-induced cytochrome C release from mitochondria is independent of the permeability transition pore but highly dependent on Mg^2+ ions [J]. Cell Biol, 1998,143 (1) :217-224.
  • 3Bemardi P. Mitochondrial transport of cations:channels, exchangers,and permeability transition[J]. Physiol Rev, 1999,79(4):1127-1155.
  • 4Short AD, Bian J, Ghosh TK, et al. Intracellrlar Ca^2+ poll content is linked to control of cell growth[J]. Proc Natl Acad Sci USA, 1993,90(11) : 4986-4990.
  • 5Scimeca JC, Servant MJ, Dyer JO, et al. Essential role of calcium in the regulation of MAP kinase phosphatase-1 extression [J]. Oncogene,1997,15(16) :717-725.
  • 6Kumaran C, Shivakumar K. Calcium- and superoxide anion-mediated mitogenic action of substance P on cardiac fibroblasts[J]. Am J Physiol Heart Circ Physiol,2002,282(5) : 1855-1862.
  • 7Chen YC, Kuo TC, Lin-Shiau SY, et al. Induction of HSP70 gene expression by modulation of Ca^2+ ion and cellular p53 protein by curcumin in colorectal carcinoma cells[J]. Mol Carcinog, 1996, 17(4):224-234.
  • 8Nakayama T, Hattori M, Uchida K, et al. The regulatory domain of the inositol 1,4,5-trisphosphate receptor is necessary to keep the channel domain closed:possible physiological significance of specific cleavage by caspase 3[J]. Biochem,2004,377(2) :299-307.
  • 9Kulkarni GV, Lee W, Seth A, et al. Role of mitochondrial membrane potential in concanavalin A-induced apoptosis in human fibroblasts[J].Exp Cell Res, 1998,245 (1) : 107-108.
  • 10Stefanovic B, Stefanovic L, Schnabl B, et al. TRAM2 protein interacts with endoplasmic reticulum Ca^2+ pump Serca2b and is necessary for collagen type Ⅰ synthesis[J]. Mol Cell Bio1,2004,24(4):1758-1768.

共引文献3

同被引文献29

  • 1Glogauer M, Ferrier J, McCulloch CA. Magnetic fields applied to collagen - coated ferric oxide beads induce strectch - activated Ca2 + flux in fibroblasts[J]. Am J Physiol, 1995,269:1093 - 1104
  • 2Arora PD, Bibby K J, McCulloch CA. Slow oscillations of free intracellular calcium ion concentration in human fibroblasts responding to mechanical stretch [J]. J Cell Physiol, 1994, 161:187 - 200
  • 3Ko KS, Arora PD, McCulloch CA. Cadherins mediate intercellular mechanical signaling in fibroblasts by activation of stretch - sensitive calcium - permeable channels [ J ]. J Biol Chem, 2001, 276:35967 - 35977
  • 4Shemesh T, Geiger B, Bershadsky AD, Kozlov MM. Focal adhesions as mechanosensors: a physical mechanism[J].Proc Natl Acad Sci U S A, 2005, 102:12383 -12388
  • 5Ohara A, Saeki Y, Nishikawa M, et al. Single - channel record- ings of TREK - 1 K + channels in periodontal ligament fibroblasts [J]. J Dent Res, 2006, 85:664 -669
  • 6Takahashi A, Ikehara T, Hosokawa K, et al. Properties of Ca^2+ - dependent K^+ channels of human gingival fibroblasts[J]. J Dent Res, 1995, 74:1507 - 1512
  • 7Chung MK, Kim HCh. Volume - activated chloride currents from human fibroblasts: blockade by nimodipine[J]. Gen Physiol Biophys, 2002, 21:85 - 101
  • 8Graber TM, Vanarsdall RL Jr. Orthodontics: current principles and techniques [ J]. 3 nd edn. St Louis: Mosby, 2000:96 - 192
  • 9Bass RB, Strop P, Barclay M, et al. Crystal structure of Escherichia coli MscS, a voltage - modulated and mechanosensitive channel [J].Science, 2002, 298:1582-1587
  • 10Von wichert G, Jiang G, Kostic A, et al. RPTP - alpha acts as a transducer of mechanical force on alphav/beta3 - integrin - cytoskeleton linkages[J].J Cell Biol, 2003, 161:143 -153

引证文献2

二级引证文献7

中国美容医学
2008年 第1期

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