亚硒酸钠对乙型肝炎病毒的体外抑制作用

Effect of sodium selenite on hepatitis B virus replication in vitro

目的探讨亚硒酸钠在体外对乙型肝炎病毒(HBV)蛋白合成、DNA复制的影响及机制。方法将不同浓度的亚硒酸钠作用于HepG2.2.15细胞系,通过检测细胞培养上清液中乙型肝炎表面抗原(HBsAg)、乙型肝炎e抗原(HBeAg)水平、乙型肝炎核心抗原(HBcAg)和HBV DNA水平来评价HBV复制情况;免疫组织化学检测HepG2.2.15细胞p53蛋白的表达情况。结果亚硒酸钠对HBV复制具有抑制作用,随着亚硒酸钠浓度的升高,对HBsAg和HBeAg的抑制率逐渐上升,但对HBsAg的抑制作用要小于HBeAg。细胞内HBV DNA复制水平也逐渐下降(P<0.01)。p53蛋白的分布也发生了改变。结论亚硒酸钠对HepG2.2.15细胞HBsAg、HBeAg和HBcAg表达、HBV DNA复制均有抑制作用,作用机制与其干扰p53蛋白的表达有关。

AIM To investigate the effect of sodium selenite on viral protein synthesis and hepatitis B virus （HBV） DNA replication in vitro. METHODS The HBV DNA transfected cell line HepG2.2.15 was treated with different concentrations of sodium selenite for 3 days. Hepatitis B surface antigen （HBsAg）, hepatitis B e antigen （HBeAg）, Hepatitis B core antigen （HBcAg） and level of HBV DNA in supernatant were detected by enzyme-linked immunosorbent assay （ELISA）. Changes of p53 were detected by immunohistochemical method in cell line HepG2.2.15. RESULTS Sodium selenite could suppress the expression of HBsAg and HBeAg, and inhibit the HBV DNA replication in a dose-dependent manner. The suppression rate of HBsAg was lesser than that of HBeAg. HBcAg under the action of sodium selenite showed similar effect on HBV DNA replication （P 〈 0.01）, with the expression change of p53. CONLUSION Sodium selenite can inhibit the expressions of HBsAg, HBeAg, HBcAg in the HepG2.2.15 all line and HBV DNA replication through interference with the possible mechanism of abnormal expression of p53 protein.