盐酸去氯羟嗪和盐酸氯丙那林的毛细管电泳-电致化学发光法同时测定

Determination of Decloxizine Hydrochloride and Clorprenaline Hydrochloride with Capillary Electrophoresis-Electrochemiluminescence

基于盐酸去氯羟嗪和盐酸氯丙那林都能增强联吡啶钌的电致化学发光信号，建立了一种分离检测海珠喘息定片中盐酸去氯羟嗪和盐酸氯丙那林的毛细管电泳-电致化学发光新方法。考察了联吡啶钉浓度、检测电位、磷酸盐缓冲液（PBS）浓度及其pH值、进样电压和进样时间等实验条件对分离、检测体系的影响。在优化的实验条件下，海珠喘息定片中的盐酸去氯羟嗪和盐酸氯丙那林在4min内可实现分离检测，其线性范围均为5×10^-7-1×10^-5mol／L（相关系数分别为0．9984和0．9990），检出限分别为1．05×10^-7mol／L和6.98×10^-9mol／L（S／N=3）。

Based on the electrochemiluminescence enhancement of tris （2, 2＇-bipyridyl）ruthenium （ Ⅱ ） with decloxizine hydrochloride（DH） and clorprenaline hydrochloride（CH）, a novel and sensitive method for the determination of DH and CH in Haizhuchuanxiding tablet was established using capillary electrophoresis （CE） coupled with electrochemiluminescence （ECL） detection. The effects of several factors such as the concentration of tris（2, 2＇-bipyridyl） ruthenium（ Ⅱ ）, the detection potential, the concentration and the pH of phosphate buffer, the electrokinetic voltage and the injection time were investigated. Under optimum conditions, DH and CH in Haizhuchuanxiding tablet could be separated and detected within 4 min. The linear concentration of DH ranged from 5.0 × 10^-7 to 1.0 × 10^-5 mol/L with a correlation coefficient of 0. 998 4 and a detection limit of 1.05 ×10 ^-7 mol/L （S/N =3）. The linear concentration of CH ranged from 5.0 ×10 ^-7 to 1. 0 × 10^-5 mol/L with a correlation coefficient of 0. 999 0 and a detection limit of 6. 98 ×10^-8 mol/L（ S/N= 3 ）.