畸胎瘤细胞源性生长因子和血管内皮生长因子在食管鳞癌中的表达及临床意义

Expression of PC cell-derived growth factor and vascular endothelial growth factor in esophageal squamous cell carcinoma and their clinicopathologic significance

目的探讨食管鳞癌(ESCC)中畸胎瘤细胞源性生长因子(PCDGF)、血管内皮生长因子(VEGF)的表达与肿瘤临床病理参数之间的关系,明确PCDGF和VEGF在血管生成中的作用。方法以免疫组化方法检测郑州大学第一附属医院2005年7月至2006年5月收治的50例食管鳞癌患者手术切除标本PCDGF与VEGF的表达,并以CD105抗体标记肿瘤组织血管内皮细胞,计算肿瘤间质微血管密度(MVD)。结果食管鳞癌中PCDGF、VEGF的表达较正常食管上皮明显增加(P<0.01);PCDGF和VEGF与肿瘤的浸润深度、TNM分期和淋巴结转移呈正相关(P均<0.05);PCDGF、VEGF的表达与MVD值呈显著正相关(P<0.01);PCDGF的表达与VEGF的表达呈正相关(P<0.05)。结论PCDGF标记癌组织的敏感性较高,有望成为一种新的食管鳞癌肿瘤标志物。食管鳞癌中PCDGF、VEGF的表达与血管生成关系密切,可能通过促进肿瘤新生血管生成参与肿瘤的生长、浸润和转移。

Objective To investigate the expression of PC cell-derived growth factor（ PCDGF or progranulin） and vascular endothelial growth factor （VEGF） and the relationships between them and clinicopathologic features in esophageal squamous cell carcinoma（ESCC） ,helping to determine the role of PCDGF（progranulin） and VEGF in angiogenesis. Methods The expression of PCDGF（progranulin） and VEGF in 50 surgical specimens from patients with ESCC and 20 with normal esophageal mueosa were detected by immunohistochemistry. The vascular endothelial eens in tumor tissue were labeled by antibody to CD105 for counting microvessel density（MVD）. Results The expressions of PCDGF（progranulin） and VEGF in ESCC were obviously higher than normal mucosa. Their expression had positive correlation with depth of tumor invasion,lymph node metastasis, and TNM classification. In ESCC, both the expression level of PCDGF （progranulin） and VEGF had significant positive correlation with MVD and the expression of PCDGF had direct correlation with the expression of VEGF. Conclusion PCDGF marks tumor tissues with so high sensitivity that it can bea novel ESCC marker hopefully. The expression of both PCDGF（progranulin） and VEGF in ESCC has close relationship with angiogenesis, and they may take part in tumor growth,infiltration and metastasis through promoting tumor angiogenesis.