二脱氧肌苷引发培养的背根神经节神经元的形态学改变(英文)

Morphological alterations induced by didanosine in cultured dorsal root ganglion nrurons

为探讨二脱氧肌苷(ddI)对培养的背根神经节(DRG)神经元的影响,我们对分散培养的胎鼠DRG神经元培养3d后,再分别以不同浓度的ddI(1μg/ml,5μg/ml,10μg/mll和20μg/ml)孵育3d。终止培养后,行微管相关蛋白2(MAP2)标记,用共聚焦激光扫描显微镜观察神经元胞体和突起的改变。结果表明,DRG神经元用ddI孵育3d,神经元突起的数目减少和长度变短,呈剂量依赖性,而神经元的直径则没有变化。本研究的结果表明,ddI可影响培养的DRG神经元突起的再生和生长。

To investigate the effects of didanosine （ ddl ） on the morphological alterations of dorsal root ganglion （DRG） neurons, dissociated DRG cells from rat embryo were studied. DRG cells were cultured for 3 days and then treated with ddl for additional 3 days in different concentrations （ 1 μg/ml, 5 μg/ml, 10 μg/ml and 20μg/ml, respectively）. After that, DRG cells were processed for microtubule associated protein 2 （ MAP2 ） labeling and observed under confecal laser scanning microscopy （ CLSM ）. The results showed that both the number and length of neurites of the DRG cells after exposed to ddl significantly down-regnlated in a dose-dependent manner compared with control group, thus suggesting that ddl may have inhibitory effects on neurite regeneratian and outgrowth in dissociated DRG cultures.