水牛唾液N-乙酰-β-D-氨基葡萄糖苷酶的变化规律与排卵

RELATIONSHIP BETWEEN SALIVARY ACTIVITY OF Nacetyl β DGLUCOSAMINIDASE AND OVULATION TIME IN SWAMP BUFFALO COWS

研究了湖北沼泽型母水牛在发情末期唾液中Ｎ－乙酰－β－Ｄ－氨基葡萄糖苷酶（ＮＡＧ）的变化与排卵的关系。结果表明，在ＮＡＧ活性峰出现前的酶单位为１８．９６±６．７３（ｎ＝１２８），临排卵前出现的活性峰的酶单位为６０．３７±１５．４６（ｎ＝１２８），两者之间的差异显著（Ｐ＜０．０１）。５头发情排卵典型的母水牛，在其发情末期唾液中ＮＡＧ的活性呈现规律性变化，在临排卵前出现一明显高峰，此前３～６ｈ尚出现一小峰。在母水牛发情末期待小峰出现而后下降时进行冷冻精液人工授精，２个冻配点的情期产犊率和受胎率分别为４５．８３％（２２／４８）和４５．００％（３６／８０）。

Chinese swamp buffalo cows were conducted to test the salivary activity of Nacetyl β Dglucosaminidase (NAG). The unit of activity of NAG during the ending of the standing heat averaged 18.96±6.73 ( ±s ). The unit of activity of NAG near the ovulation time reached a peak value with an average of 60.37±15.46 ( ±s ). The difference was singnificent ( P <0.01). The curves of NAG variation were drawn in 5 buffalo cows. The ovulation accurred at 2 h after the peak reached. A subpeak accurred before 3 h to 6 h of the peak. Tested buffalo cows were inseminated artificially with frozen semen from Riverine buffalo bull at the time when the subpeak had declined, the calving rate and pregnancy rate to the first insemination was 45.83% (2248) and 45.00% (3680) respectively.