猪肌注和鸡口服烟酸诺氟沙星后的药物动力学研究

PHARMACOKINETICS OF NORFLOXACIN NICOTINATE IN PIGS AFTER INTRAMUSCULAR ADMINISTRATION AND IN CHICKENS AFTER ORAL ADMINISTRATION

湖北白猪８头单次肌内注射烟酸诺氟沙星１０ｍｇ／ｋｇ后，利用高效液相色谱法分析血浆中诺氟沙星的浓度，其检测限可达２５ｎｇ／ｍｌ血浆。房室模型分析表明，其吸收、分布和消除半衰期（Ｔ１２Ｋａ、Ｔ１２α、Ｔ１２β分别为（０．０４３４±０．０１１９）ｈ、（０．３０７８±０．１２２１）ｈ和（３．３４４６±０．１８８９）ｈ，最大血药浓度（Ｃｍａｘ）为（２．３６８０±０．４１１９）μｇ／ｍｌ，达峰时间（Ｔｍａｘ）为（０．２７±０．０４）ｈ，稳态血药浓度（Ｃｓ，τ＝１２ｈ）为（０．７０７±０．１２７）μｇ／ｍｌ。ＡＡ肉鸡８只口服烟酸诺氟沙星１０ｍｇ／ｋｇ后，药物在体内呈现单室模型过程，其吸收半衰期和消除半衰期（Ｔ１２Ｋａ、Ｔ１２Ｋｅ）分别为（０．８２３２±０．４０６２）ｈ和（３．４９７７±０．２８２３）ｈ，Ｃｍａｘ为（０．２２０９±０．０４０６）μｇ／ｍｌ，Ｔｍａｘ为（２．３１５９±０．６６３８）ｈ，平均稳态血浆药物浓度（Ｃｓ，τ＝１２ｈ）为（０．１３８０±０．０２３５）μｇ／ｍｌ。根据其药物动力学特征参数制订了猪肌注和鸡口服烟酸诺氟沙星的给药方案。

Pharmacokinetic variables of a new quinolone antimicrobial, norfloxacin nicotinate, were studied in Hubei white pigs and chickens. The drug was given to 8 healthy pigs at a dosage of 10 mg per kilogram of body weight intramuscularly and to 8 healthy chickens at a dosage of 10 mg per kilogram of body weight orally. Plasma norfloxacin nicotinate concentrations were determined by using high performance liquid chromatography, with a limit of detection of 25 ng of norfloxacin nicotinateml of plasma. Plasma norfloxacin nicotinate pharmacokinetic variables were calculated by using a twocompartment open model in pigs and a onecompartment open model in chickens, with absorption, distribution and elimination halflives of (0.0434± 0.0119) h, (0.3078±0.1221) h and (3.3446±0.1889) h ( ±s ) respectively in pigs and absorptioin, eliminatoin halflives of (0.8232±0.4062) h and (3.4977±1.2126) h respectively in chickens. The maximal plasma concentration was (2.3680±0.4119) μgml, peaking for (0.2667±0.0402) h after dosing the drug to pigs; and (0.2209±0.0406) μgml, peaking for (2.3159±0.6638) h after dosing the drug to chickens. The dosage regiments of the drug for pigs and chickens were calculated based on this study.