夜交藤药材RP-HPLC色谱指纹图谱研究与评价

STUDIES ON THE FINGERPRINT FOR CAULIS POLYGONI MULTIFLORI

目的建立夜交藤药材的反相高效液相色谱(reversed phase-high performance liquid chromatography,RP-HPLC)色谱指纹图谱分析方法,为有效控制和科学评价夜交藤药材质量提供新方法。方法夜交藤用乙酸乙酯超声处理,提取液采用RP-HPLC法进行测定。DiamonsilTMC18色谱柱(250 mm×4.6 mm,5μm),乙腈-0.1%磷酸梯度洗脱,检测波长290 nm,流速1 mL/min,柱温30℃。结果建立了夜交藤药材RP-HPLC色谱指纹图谱的共有模式,并对不同产地药材进行相似度比较与评价,精密度、重复性与稳定性实验中各共有峰相对峰面积和相对保留时间的相对标准偏差均小于5.0%,符合指纹图谱的技术要求。结论该方法简便、可靠,为夜交藤药材的鉴别和质量控制提供了依据,可作为不同产地夜交藤药材的质量评价标准。

Objective To establish reversed phase-high performance liquid chromatography （RP-HPLC） fingerprint for control the quality of Caulis Polygoni Multiflori. Methods Caulis Polygoni Multiflori was extracted by supersonic wave with ethyl acetate. The chromatographic procedure for fingerprint was carried out using Diamonsil^TM C18 as an analytical column gradient eluted with a mixture consisting of acetonitrile and 0.1% phosphoric acid. The detection wave length was 290 nm. The flow rate was 1 mL/min and the column tempreture was 30 centigrade. Results The mutual mode of RP-HPLC fingerprint was set up and the similar degrees of the crude drugs from different places were compared. The RSD of relative peak areas and retention time of common peak in precision, repeatability,and stability did not reach 5.0%. Conclusion This simple and reliable method can be used for the fingerprint assay and quality control for Caulis Polygoni Multiflori. It provides a new method as a comprehension quality control item for Caulis Polygoni Multiflori.