母婴垂直传播沙眼衣原体的聚合酶链反应及测序研究

A study on the vertical transmission of chlamydia trachomatis with PCR and DNA sequencing

为从分子水平探讨重庆母婴垂直传播沙眼衣原体的发生情况，采用ＭｃＣｏｙ细胞培养及聚合酶链反应技术（ＰＣＲ）检测沙眼衣原体（ＣＴ），并对一母亲宫颈标体及其婴儿鼻咽标本ＰＣＲ扩增出的ＤＮＡ片段克隆后测序。结果：孕妇宫颈ＣＴ培养１０．８％（３０／２７８）阳性，ＰＣＲ阳性率１４．０％（３９／２７８）；母婴垂直传播ＣＴ阳性率５５．０％（１１／２０）；一对母婴标本ＣＴ的ＤＮＡ片段序列完全一致。提示：首次从ＤＮＡ测序分子水平证实母婴垂直传播ＣＴ，垂直传播ＣＴ在重庆地区颇为常见。

To study the vertical transmission of C. trachomatis (CT) in Chongqing, the authors applied endocervical swabs of 278 pregnant women and nasopharyngeal and conjunctival swabs of 79 babies for isolation of CT in McCoy cell culture. The sequence of PCR amplified DNA fragment of CT from the cervix of a mother and the nasopharynx of her baby was determined. The results showed that 10.8% of the cervical swabs were positive for CT culture, while the positive rate of the PCR was 14.0%. The vertical transimission rate of CT was 55.0%. The sequences of amplified DNA fragments of CT from a maternal infant pair matched perfectly. This is the first report of vertical transmission of CT proved by DNA sequencing.