转反义GVDE基因烟草的叶绿素荧光参数及抗氧化酶活性的变化

Changes of Chlorophyll Fluorescence Parameters and Antioxidative Enzymes Activities in Antisense GVDE Tobacco

【目的】研究姜紫黄质脱环氧化酶基因(GVDE)的功能。【方法】以烟草为试材,构建反义抑制表达载体。将从姜中克隆的GVDE基因反向转入烟草,对获得的抗性植株用PCR和PCR-Southern检测分析。同时测定烟草叶片荧光参数变化和抗氧化物酶(APX、SOD、POD)活性。【结果】反义基因已整合到烟草的基因组中。转反义GVDE基因烟草的NPQ、Fv/Fm始终低于对照,APX、SOD和POD等抗氧化酶活性也低于对照植株。【结论】在转反义GVDE基因的烟草中,强光下耗散过剩光能的非辐射能量耗散能力明显降低。

[ Objective ] In order to research the function of GVDE gene which was cloned from ginger leaves. [ Method ] Using tobacco plants as material, an antisense expression GVDE vector to transform tobacco leaves was constructed. Transgenic assays were performed using PCR and PCR-Southem. At the same time the changes of chlorophyll fluorescence parameters and antioxidative enzymes were measured. [Result] PCR amplification and PCR-Southem demonstrated that this antisense gene was successfully integrated into tobacco genome. NPQ, Fv/Fm in transgenic tobacco were lower than in CK under high light, and the activities of APX, SOD and POD in transgenic tobacco were also lower than in CK. [Conclusion] The ability of radiationless energy dissipation decreased evidently in transgenic tobacco under high light.