基于离散小波变换的膜蛋白跨膜螺旋区段预测

Prediction of Transmembrane Helical Segments in Membrane Proteins Based on Discrete Wavelet Transform

基因组计划所产生的大量蛋白质序列迫切需要从理论上预测跨膜螺旋区段.文中采用基于离散小波变换的方法预测膜蛋白跨膜螺旋区段的数目和位置.以PDB代码为1KQG的膜蛋白为例,描述了该方法对跨膜螺旋区段的数目和位置的预测分析过程.从最新的MPtopo数据库中随机抽取80条三维结构已知的膜蛋白序列作为测试集(含325个跨膜螺旋区),采用该方法对跨膜螺旋区段进行预测,其中312个跨膜螺旋区被准确预测到,预测准确率为96.6%.并将预测结果与7种常用预测方法的主要预测结果进行了比较.结果表明,该文提出的方法具有较高的预测准确度.

Increasing protein sequences from the genome project require theoretical methods to predict transmembrane helical segments （TMHs）. In this paper, a method based on discrete wavelet transform （DWT） is developed to predict the number and location of TMHs in membrane proteins. PDB coded as 1KQG is chosen as an example to describe the prediction process with this method. Eighty proteins with known 3D-structure from the Mptopo database are chosen at random as the test objects （including 325 TMHs）, 312 of which can be predicted and the prediction accuracy is 96. 6%. Compared with main prediction results of seven common prediction methods, the obtained results indicate that the proposed method has higher prediction accuracy.