还原型谷胱甘肽对大鼠肺不同潮气量机械通气保护作用

Effects of reduced glutathione on different tidal volume mechanical ventilation injury in the rat lung

目的探讨还原型谷胱甘肽对大鼠不同潮气量机械通气保护作用。方法将60只健康雄性SD大鼠随机分成对照组（A组）、正常通气组（B组）、过度通气组（C组）、正常通气组＋还原型谷胱甘肽（D组）、过度通气组＋还原型谷胱甘肽（E组），D，E组在机械通气前5min阴茎背静脉注射还原型谷胱甘肽，各组大鼠通气时间均为4h。观察各组肺组织的病理改变，用干／湿重比值来比较肺水肿的程度，用免疫组织化学技术检测肺组织核转录因子NF-κB的表达，ELISA法检测肺匀浆中肿瘤坏死因子TNF-α变化。结果病理学改变C组与A、B、D，E组比较的肺组织有更多白细胞浸润和肺泡壁结构的破坏。C组肺组织中TNF-α含量与D、E比较明显增加，C组肺组织NF-κB的表达与D、E组比较明显增加。结论还原型谷胱甘肽对大鼠不同潮气量机械通气损伤的保护作用。

Objective To study the protective effects of reduced glutathione on rats under different tidal volume ventilations. Methods Sixty SD rats were randomly divided into five groups： Control group （group A）, normal ventilation group （group B） hyperventilation group （group C）, normal ventilation plus reduced glutathione group （group D） and hyperventilation plus reduced glutathione group （group E）. Rats in group D and E were injected with reduced glutathione through vena dorsalis penis 5 minutes before mechanical ventilation. The duration of ventilation was 4 hours for each group. Lung biopsies were used for examination of dry/wet ratio to compare the levels of lung edema. The NF-κB expression in pulmonary endothelium was assessed by immunohistochemical staining. The level of tumor necrosis factor alpha （TNF-α） was detected by ELISA. Results Lung biopsies of rats in group C showed more leukocytic infiltrate, pulmonary capillary congestion, interstital edema and interalveolar hemorrhage than those of other groups. The expressions of the NF-κB and TNF-α of .group C were significantly higher than those of other groups. Conclusions Reduced glutathione could protect rats＇lung under different tidal volume ventilation injury.