孕妇HBV感染与胎盘组织TLR3表达关系

Relationship between TLR3 expression in placenta and hepatitis B virus infection in pregnant women

目的了解Toll样受体3（TLR3）与孕妇乙型肝炎病毒（船v）感染的关系。方法采用半定量逆转录一聚合酶链反应（RT—PCR）技术和免疫组织化学ABe法分别检测正常孕妇和HBsAg阳性孕妇胎盘组织中TLR3mR—NA和TLR3受体，从基因和蛋白水平比较TLR3在2组表达和分布差异。结果半定量RT—PCR结果显示，病例组TLR3 mRNA的含量低于对照组，吸光度比值（TLR3／β—actin）分别为0．4175±0．2216，0．6387±0．2053，2组比较差异有统计学意义（t=3．003，P〈0．05）；免疫组织化学（ABC）结果显示，TLR3受体在对照组阳性率为100％（41／41），病例组阳性率为73．0％（85／116），2组比较差异有统计学意义（x^2=5．641，P〈0．05）。结论胎盘组织TLR3表达与孕妇HBV感染有一定关系；TLR3高表达是孕妇HBV感染的保护因素（OR=0．733，95％CI：0．656～0．818）。

Objective To explore the relationship of TLR3 in placenta and hepatitis B virus infection in pregnant women through comparing the difference of TLR3 mRNA expression and protein distribution in normal and HBsAg positive pregnant women. Methods 157 placenta of normal and HBsAg positive pregnant women were collected. Expression of TLR3 mRNA was analyzed by reverse transcriptase PCR（RT- PCR）. Localization of TLR3 protein expression in placenta was analyzed with immunohistochemistry ABC technology. Results RT - PCR revealed that TLR3 decreased in gene levels in HBV infection women＇s placenta（ t = 3.003, P 〈 0.05 ）. Expression of TLR3 protein in paraffin - embedded placenta tissue indicated expression was lower in HBV infection women than in normal women（x^2 = 5.641, P 〈 0.05）. TLR3 protein can be expressed on all section of placenta. Conclusion The down - regulation of TLR3 associates with HBV infection in pregnant women. TLR3 perhaps can effectively protect placenta from HBV infection（ OR = 0. 733, 95% CI = 0. 656- 0.818）.